AIMS: The purpose of the study was to examine the effect of 1, 25(OH)₂ Vitamin D₃ supplementation on type 2 diabetic (T2DM) mice. MATERIALS AND METHODS: A total of 24 mice were taken and divided into three groups of control; diabetic and diabetic + vitamin D supplemented ones. Serum calcium level, fasting blood glucose level (FBG), hexokinase activity, glucose-6-phosphatse and fructose 1,6 bisphosphatase activity were measured to establish a relevant correlation between vitamin D supplementation and hyperglycemia in T2DM. RESULTS: There occurred an increase in FBG levels (250 ± 0.41 mg/dl) and a significant decrease in serum calcium levels in the diabetic group (8.63 ± 0.40 mg/ml) both of which reached near control levels on vitamin D₃ supplementation. The activity of the glucose metabolic enzymes was also assayed in diabetic group and was found to be deviated from control group; hexokinase (0.0241 ± 0.014 μg/mg/ml) FBPase (0.433 ± 0.002 μg/mg/ml) and G6Pase (0.918 ± 0.02 μg/mg/ml). However, the activity of these enzymes returned to near control values with hexokinase activity reaching 0.717 ± 0.003 μg/mg/ml on vitamin D₃ supplementation. The FBPase and G6Pase activities were decreased to 0.2733 ± 0.008 μg/mg/ml and G6Pase 0.71 ± 0.01 μg/mg/ml respectively. In addition to enzymatic analysis, the organs of all three groups of mice were subjected to comet assay. The diabetic group receiving vitamin D supplementation showed a marked recovery exhibiting shorter tail length both in liver (21.80 ± 2.40 μm) and pancreatic cells (19.25 ± 1.90 μm) as compared to the diabetic group exhibiting a tail length of 30.41 ± 2.50 μm and 32.45 ± 2.87 μm in liver and pancreatic cells respectively. CONCLUSION: The present study shows that vitamin D₃ supplementation is positively correlated with decrease in blood glucose level and serum calcium level in fasting condition. This suggests a positive influence of vitamin D on glucose homeostasis. Besides, the activity of various glucose metabolic enzymes (hexokinase, FBPase and G6Pase) as shown by our results and the remarkable shortening of DNA tail length in vitamin D supplemented diabetic group as compared to diabetic group without supplementation further support the idea that vitamin D supplementation might be an add-on therapy for patients with T2DM.
AIMS: The purpose of the study was to examine the effect of 1, 25(OH)₂ Vitamin D₃ supplementation on type 2 diabetic (T2DM) mice. MATERIALS AND METHODS: A total of 24 mice were taken and divided into three groups of control; diabetic and diabetic + vitamin D supplemented ones. Serum calcium level, fasting blood glucose level (FBG), hexokinase activity, glucose-6-phosphatse and fructose 1,6 bisphosphatase activity were measured to establish a relevant correlation between vitamin D supplementation and hyperglycemia in T2DM. RESULTS: There occurred an increase in FBG levels (250 ± 0.41 mg/dl) and a significant decrease in serum calcium levels in the diabetic group (8.63 ± 0.40 mg/ml) both of which reached near control levels on vitamin D₃ supplementation. The activity of the glucose metabolic enzymes was also assayed in diabetic group and was found to be deviated from control group; hexokinase (0.0241 ± 0.014 μg/mg/ml) FBPase (0.433 ± 0.002 μg/mg/ml) and G6Pase (0.918 ± 0.02 μg/mg/ml). However, the activity of these enzymes returned to near control values with hexokinase activity reaching 0.717 ± 0.003 μg/mg/ml on vitamin D₃ supplementation. The FBPase and G6Pase activities were decreased to 0.2733 ± 0.008 μg/mg/ml and G6Pase 0.71 ± 0.01 μg/mg/ml respectively. In addition to enzymatic analysis, the organs of all three groups of mice were subjected to comet assay. The diabetic group receiving vitamin D supplementation showed a marked recovery exhibiting shorter tail length both in liver (21.80 ± 2.40 μm) and pancreatic cells (19.25 ± 1.90 μm) as compared to the diabetic group exhibiting a tail length of 30.41 ± 2.50 μm and 32.45 ± 2.87 μm in liver and pancreatic cells respectively. CONCLUSION: The present study shows that vitamin D₃ supplementation is positively correlated with decrease in blood glucose level and serum calcium level in fasting condition. This suggests a positive influence of vitamin D on glucose homeostasis. Besides, the activity of various glucose metabolic enzymes (hexokinase, FBPase and G6Pase) as shown by our results and the remarkable shortening of DNA tail length in vitamin D supplemented diabetic group as compared to diabetic group without supplementation further support the idea that vitamin D supplementation might be an add-on therapy for patients with T2DM.
Authors: Gillian E Walker; Roberta Ricotti; Marta Roccio; Stefania Moia; Simonetta Bellone; Flavia Prodam; Gianni Bona Journal: PLoS One Date: 2014-01-03 Impact factor: 3.240
Authors: Stephanie R Sisley; Deanna M Arble; Adam P Chambers; Ruth Gutierrez-Aguilar; Yanlin He; Yong Xu; David Gardner; David D Moore; Randy J Seeley; Darleen A Sandoval Journal: Diabetes Date: 2016-05-23 Impact factor: 9.461