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Literature DB >> 2277064

Actin mRNA localizes in the absence of protein synthesis.

Abstract

Actin mRNA is localized in chicken embryo fibroblasts to the distal regions of leading lamellae, but not within the ruffling edges. In this investigation we have addressed the role of actin translation in this process. The translocation of actin mRNA to the cell periphery was studied by monitoring the distribution of actin mRNA in cells during spreading. Within 90 min, actin mRNA moved from a perinuclear to a peripheral distribution. Formation of lamellipodia preceded actin mRNA localization, indicating that localization is not a prerequisite for this event. Neither puromycin (which dissociates ribosomes from mRNA) nor cycloheximide (which stabilizes ribosomes on mRNA) had any effect on this movement of actin mRNA. Anchoring of actin mRNA was studied using cells with peripherally localized actin mRNA. No change in actin mRNA localization was observed for 30 min in the same inhibitors. These data indicate that the presence of the nascent polypeptide is not necessary for translocation of actin mRNA to the cell periphery, or anchoring at that site. This suggests that the mRNA contains information concerning its spatial distribution within the cytoplasm.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1990 PMID： 2277064 PMCID： PMC2116407 DOI： 10.1083/jcb.111.6.2397

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

31 in total

1. INHIBITION BY PUROMYCIN OF AMINO ACID INCORPORATION INTO PROTEIN.

Authors: M B Yarmolinsky; G L Haba
Journal: Proc Natl Acad Sci U S A Date: 1959-12 Impact factor: 11.205

2. Cotranslational assembly of myosin heavy chain in developing cultured skeletal muscle.

Authors: W B Isaacs; A B Fulton
Journal: Proc Natl Acad Sci U S A Date: 1987-09 Impact factor: 11.205

3. The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes.

Authors: J K Yisraeli; D A Melton
Journal: Nature Date: 1988-12-08 Impact factor: 49.962

4. Selective localization of messenger RNA for cytoskeletal protein MAP2 in dendrites.

Authors: C C Garner; R P Tucker; A Matus
Journal: Nature Date: 1988-12-15 Impact factor: 49.962

5. Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network.

Authors: G Frigerio; M Burri; D Bopp; S Baumgartner; M Noll
Journal: Cell Date: 1986-12-05 Impact factor: 41.582

6. Studies on the genesis of polyribosomes. I. Origin and significance of the subribosomal particles.

Authors: W K Joklik; Y Becker
Journal: J Mol Biol Date: 1965-09 Impact factor: 5.469

7. cis-acting sequences responsible for anterior localization of bicoid mRNA in Drosophila embryos.

Authors: P M Macdonald; G Struhl
Journal: Nature Date: 1988-12-08 Impact factor: 49.962

8. Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected HeLa cells.

Authors: M Cervera; G Dreyfuss; S Penman
Journal: Cell Date: 1981-01 Impact factor: 41.582

9. The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophila embryo.

Authors: T Berleth; M Burri; G Thoma; D Bopp; S Richstein; G Frigerio; M Noll; C Nüsslein-Volhard
Journal: EMBO J Date: 1988-06 Impact factor: 11.598

10. Ultrastructural visualization of cytoskeletal mRNAs and their associated proteins using double-label in situ hybridization.

Authors: R H Singer; G L Langevin; J B Lawrence
Journal: J Cell Biol Date: 1989-06 Impact factor: 10.539

34 in total

Actin mRNA localizes in the absence of protein synthesis.

1. INHIBITION BY PUROMYCIN OF AMINO ACID INCORPORATION INTO PROTEIN.

2. Cotranslational assembly of myosin heavy chain in developing cultured skeletal muscle.

3. The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes.

4. Selective localization of messenger RNA for cytoskeletal protein MAP2 in dendrites.

5. Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network.

6. Studies on the genesis of polyribosomes. I. Origin and significance of the subribosomal particles.

7. cis-acting sequences responsible for anterior localization of bicoid mRNA in Drosophila embryos.

8. Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected HeLa cells.

9. The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophila embryo.

10. Ultrastructural visualization of cytoskeletal mRNAs and their associated proteins using double-label in situ hybridization.

1. Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes.

Review 2. Cis-acting determinants of asymmetric, cytoplasmic RNA transport.

Review 3. Interaction between mRNA, ribosomes and the cytoskeleton.

4. Myosin heavy-chain mRNA is present in both myofibrillar and subsarcolemmal regions of muscle fibres.

5. Resorption-cycle-dependent polarization of mRNAs for different subunits of V-ATPase in bone-resorbing osteoclasts.

6. Characterization of a beta-actin mRNA zipcode-binding protein.

Review 7. Regulation of actin isoforms in cellular and developmental processes.

8. Transgenic expression of embryonic MAP2 in adult mouse brain: implications for neuronal polarization.

9. Vimentin mRNA location changes during muscle development.

10. The transport of prolamine RNAs to prolamine protein bodies in living rice endosperm cells.