INTRODUCTION: Tuberculosis (TB) control is linked to the availability of qualified methods for microbiological diagnostics; however, microscopy with limited sensitivity is the only method available in many locations. The objective of this study was to evaluate the introduction of culture, drug susceptibility testing (DST), and genotyping in the routine of a Municipal Program of Tuberculosis Control. METHODS: Direct microscopy of sputum and culture in Ogawa-Kudoh were performed on 1,636 samples from 787 patients. DST of positive cultures was performed by resazurin microtiter assay and genotyping by mycobacterial interspersed repetitive units-variable number tandem repeat. RESULTS: A total 91 patients with TB were identified. The culture increased case detection by 32% compared with the microscopy; acquired resistance was 3.3% and the genotyping showed high genetic diversity. CONCLUSIONS: Ogawa-Kudoh contributed significantly to the increase in case detection and is suitable for implementation in poor-resource locations. The acquired resistance rate was lower than that reported in a recent Brazilian survey. The high genetic diversity is possibly related to the high TB prevalence in the population, as well as to early detection and suitable treatment of patients. The interaction between research and health care is important for reorienting the practice, transferring technology, and improving TB control.
INTRODUCTION:Tuberculosis (TB) control is linked to the availability of qualified methods for microbiological diagnostics; however, microscopy with limited sensitivity is the only method available in many locations. The objective of this study was to evaluate the introduction of culture, drug susceptibility testing (DST), and genotyping in the routine of a Municipal Program of Tuberculosis Control. METHODS: Direct microscopy of sputum and culture in Ogawa-Kudoh were performed on 1,636 samples from 787 patients. DST of positive cultures was performed by resazurin microtiter assay and genotyping by mycobacterial interspersed repetitive units-variable number tandem repeat. RESULTS: A total 91 patients with TB were identified. The culture increased case detection by 32% compared with the microscopy; acquired resistance was 3.3% and the genotyping showed high genetic diversity. CONCLUSIONS: Ogawa-Kudoh contributed significantly to the increase in case detection and is suitable for implementation in poor-resource locations. The acquired resistance rate was lower than that reported in a recent Brazilian survey. The high genetic diversity is possibly related to the high TB prevalence in the population, as well as to early detection and suitable treatment of patients. The interaction between research and health care is important for reorienting the practice, transferring technology, and improving TB control.
Authors: Heloisa da Silveira Paro Pedro; Susilene Maria Tonelli Nardi; Maria Izabel Ferreira Pereira; Rosângela Siqueira Oliveira; Philip Noel Suffys; Harrison Magdinier Gomes; Amanda Juliane Finardi; Eloise Brasil de Moraes; Ida Maria Foschiani Dias Baptista; Ricardo Luiz Dantas Machado; Lilian Castiglioni Journal: Mem Inst Oswaldo Cruz Date: 2015-03-31 Impact factor: 2.743
Authors: Ana Julia Reis; Simone Maria Martini de David; Luciana de Souza Nunes; Andreia Rosane de Moura Valim; Lia Gonçalves Possuelo Journal: J Bras Pneumol Date: 2016 Jul-Aug Impact factor: 2.624
Authors: Emilyn Costa Conceição; Richard Steiner Salvato; Karen Machado Gomes; Arthur Emil Dos Santos Guimarães; Marília Lima da Conceição; Ricardo José de Paula Souza E Guimarães; Abhinav Sharma; Ismari Perini Furlaneto; Regina Bones Barcellos; Valdes Roberto Bollela; Lívia Maria Pala Anselmo; Maria Carolina Sisco; Cristina Viana Niero; Lucilaine Ferrazoli; Guislaine Refrégier; Maria Cristina da Silva Lourenço; Harrison Magdinier Gomes; Artemir Coelho de Brito; Marcos Catanho; Rafael Silva Duarte; Philip Noel Suffys; Karla Valéria Batista Lima Journal: Mem Inst Oswaldo Cruz Date: 2021-03-15 Impact factor: 2.743