OBJECTIVE: To investigate the correlation between HBV infection and interleukin 27 (IL-27). METHODS: Serum IL-27 levels in patients with HBV infection and in healthy individuals were measured by ELISA, regulation of IL-27 by HBV in vitro were analyzed by RT-PCR and ELISA. RESULTS: IL-27 was significantly elevated in patients as compared to healthy individuals (P<0.001). IL-27 was also detected at higher levels in patients with liver cirrhosis or hepatocellular carcinoma than those with acute hepatitis B or chronic hepatitis B (P<0.05). We also found that IL-27 expression was influenced by HBV e antigen. In addition, our in vitro studies demonstrated that mRNA and protein expression were all stimulated in cells transfected with infectious HBV clone. CONCLUSIONS: IL-27 would be a novel cytokine for clinical management of HBV-infected patients, which may establish novel HBV therapeutic approaches.
OBJECTIVE: To investigate the correlation between HBV infection and interleukin 27 (IL-27). METHODS: Serum IL-27 levels in patients with HBV infection and in healthy individuals were measured by ELISA, regulation of IL-27 by HBV in vitro were analyzed by RT-PCR and ELISA. RESULTS:IL-27 was significantly elevated in patients as compared to healthy individuals (P<0.001). IL-27 was also detected at higher levels in patients with liver cirrhosis or hepatocellular carcinoma than those with acute hepatitis B or chronic hepatitis B (P<0.05). We also found that IL-27 expression was influenced by HBV e antigen. In addition, our in vitro studies demonstrated that mRNA and protein expression were all stimulated in cells transfected with infectious HBV clone. CONCLUSIONS:IL-27 would be a novel cytokine for clinical management of HBV-infectedpatients, which may establish novel HBV therapeutic approaches.
Authors: Marwa S Abdel-Tawab; Hanan H Fouad; Dalia A Omran; Aml E Abdou; Shaimaa Mohamed Zaied; Alaa A Mohamed Journal: Biomed Res Int Date: 2020-12-15 Impact factor: 3.411