| Literature DB >> 22765653 |
Haiyang Yu1, Yuan Siang Lui, Sijing Xiong, Wen Shing Leong, Feng Wen, Himawan Nurkahfianto, Sravendra Rana, David Tai Leong, Kee Woei Ng, Lay Poh Tan.
Abstract
We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2 kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β(3) integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering.Entities:
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Year: 2012 PMID： 22765653 PMCID： PMC3528092 DOI： 10.1089/scd.2012.0160
Source DB: PubMed Journal: Stem Cells Dev ISSN： 1547-3287 Impact factor: 3.272