PROBLEM: Although high maternal mortality was reported in the most recent pandemic of swine-origin influenza A H1N1/09 (H1N1/09), its direct effects on the feto-placental unit are unknown. In the present study, we examined the susceptibility of immortalized human trophoblasts to clinical isolates of pandemic 2009 H1N1 influenza A (H1N1/09) virus. METHOD OF STUDY: The H1N1/09 virus was isolated from a patient with influenza, sequenced and identified as the A/Narita/2009 (H1N1) strain. The trophoblast cell lines Swan71 and HTR8 were challenged with the virus and examined for the expression of H1N1/09 viral RNA and proteins. RESULTS: Viral RNA and proteins were observed 24 hr after inoculation. However, viral release was not detected. CONCLUSION: First trimester human trophoblast cell lines were susceptible to the H1N1/09 influenza A virus. However, viral release and cytopathic effects were minimal. Our data suggest that placental damage by the H1N1/09 virus may be limited.
PROBLEM: Although high maternal mortality was reported in the most recent pandemic of swine-origin influenza A H1N1/09 (H1N1/09), its direct effects on the feto-placental unit are unknown. In the present study, we examined the susceptibility of immortalized human trophoblasts to clinical isolates of pandemic 2009 H1N1influenza A (H1N1/09) virus. METHOD OF STUDY: The H1N1/09 virus was isolated from a patient with influenza, sequenced and identified as the A/Narita/2009 (H1N1) strain. The trophoblast cell lines Swan71 and HTR8 were challenged with the virus and examined for the expression of H1N1/09 viral RNA and proteins. RESULTS: Viral RNA and proteins were observed 24 hr after inoculation. However, viral release was not detected. CONCLUSION: First trimester human trophoblast cell lines were susceptible to the H1N1/09 influenza A virus. However, viral release and cytopathic effects were minimal. Our data suggest that placental damage by the H1N1/09 virus may be limited.
Authors: Elizabeth Q Littauer; E Stein Esser; Olivia Q Antao; Elena V Vassilieva; Richard W Compans; Ioanna Skountzou Journal: PLoS Pathog Date: 2017-11-27 Impact factor: 6.823
Authors: Paulina Fuentes-Zacarías; Jose M Murrieta-Coxca; Ruby N Gutiérrez-Samudio; Astrid Schmidt; Andre Schmidt; Udo R Markert; Diana M Morales-Prieto Journal: Biochim Biophys Acta Mol Basis Dis Date: 2021-07-24 Impact factor: 6.633