Steveen Rios Ribeiro1, Cinthia Furst. 1. Núcleo de Doenças Infecciosas, Universidade Federal de Espírito Santo, Vitória, ES, Brasil.
Abstract
INTRODUCTION: Spontaneous sedimentation is an important procedure for stool examination. A modification of this technique using conical tubes was performed and evaluated. METHODS: Fifty fecal samples were processed in sedimentation glass and in polypropylene conical tubes. Another 50 samples were used for quantitative evaluation of protozoan cysts. RESULTS: Although no significant differences occurred in the frequency of protozoa and helminths detected, significant differences in protozoan cyst counts did occur. CONCLUSIONS: The use of tube predicts a shorter path in the sedimentation of the sample, increases concentration of parasites for microscopy analysis, minimizes the risks of contamination, reduces the odor, and optimizes the workspace.
INTRODUCTION: Spontaneous sedimentation is an important procedure for stool examination. A modification of this technique using conical tubes was performed and evaluated. METHODS: Fifty fecal samples were processed in sedimentation glass and in polypropylene conical tubes. Another 50 samples were used for quantitative evaluation of protozoan cysts. RESULTS: Although no significant differences occurred in the frequency of protozoa and helminths detected, significant differences in protozoan cyst counts did occur. CONCLUSIONS: The use of tube predicts a shorter path in the sedimentation of the sample, increases concentration of parasites for microscopy analysis, minimizes the risks of contamination, reduces the odor, and optimizes the workspace.
Authors: Lee Hakami; Paul M Castle; Jaydon Kiernan; Koeun Choi; Anjanirina Rahantamalala; Emma Rakotomalala; Rado Rakotoarison; Patricia Wright; Simon Grandjean Lapierre; Ivan Crnosija; Peter Small; Ines Vigan-Womas; Luis A Marcos Journal: Pathog Glob Health Date: 2019-03-17 Impact factor: 2.894