INTRODUCTION: Manaus, the capital city of the state of Amazon with nearly 2 million inhabitants, is located in the middle of the Amazon rain forest and has suffered dengue outbreaks since 1998. METHODS: In this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), aimed at identifying dengue virus serotypes. RESULTS: Acute phase sera from 432 patients were tested for the presence of dengue virus. Out of the 432 patients, 137 (31.3%) were found to be positive. All the four dengue virus serotypes were observed. CONCLUSIONS: The simultaneous circulation of the four dengue serotypes is described for the first time in Manaus and in Brazil.
INTRODUCTION: Manaus, the capital city of the state of Amazon with nearly 2 million inhabitants, is located in the middle of the Amazon rain forest and has suffered dengue outbreaks since 1998. METHODS: In this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), aimed at identifying dengue virus serotypes. RESULTS: Acute phase sera from 432 patients were tested for the presence of dengue virus. Out of the 432 patients, 137 (31.3%) were found to be positive. All the four dengue virus serotypes were observed. CONCLUSIONS: The simultaneous circulation of the four dengue serotypes is described for the first time in Manaus and in Brazil.
Authors: Maria C Anholeti; Rodrigo C Duprat; Maria R Figueiredo; Maria Ac Kaplan; Marcelo Guerra Santos; Marcelo S Gonzalez; Norman A Ratcliffe; Denise Feder; Selma R Paiva; Cicero B Mello Journal: Mem Inst Oswaldo Cruz Date: 2015-07-17 Impact factor: 2.743
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Authors: Tiago F Póvoa; Ada M B Alves; Carlos A B Oliveira; Gerard J Nuovo; Vera L A Chagas; Marciano V Paes Journal: PLoS One Date: 2014-04-15 Impact factor: 3.240
Authors: Valquiria do Carmo Alves Martins; Michele de Souza Bastos; Rajendranath Ramasawmy; Regina Pinto de Figueiredo; João Bosco Lima Gimaque; Wornei Silva Miranda Braga; Mauricio Lacerda Nogueira; Sergio Nozawa; Felipe Gomes Naveca; Luiz Tadeu Moraes Figueiredo; Maria Paula Gomes Mourão Journal: PLoS One Date: 2014-06-30 Impact factor: 3.240