Kah Jing Lim1, Kda Rajan, Charles G Eberhart. 1. Department of Pathology, Johns Hopkins University School of Medicine, 720 Rutland Ave, Ross Building 558, Baltimore, MD 21205, USA.
Abstract
BACKGROUND/AIM: Zeng Sheng Ping (ZSP) is a traditional herbal remedy used to prevent progression and growth of neoplastic lesions. It has been shown to inhibit Notch2 expression in a murine lung cancer model, leading us to investigate its therapeutic potential in Notch-dependent brain tumors. MATERIALS AND METHODS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS), apoptosis, and quantitative real-time polymerase chain reaction (RT-PCR) analyses were performed in glioma and medulloblastoma cell lines, and morphological analyses in DAOY flank xenografts. RESULTS: ZSP inhibited brain tumor growth in vitro, in part, by apoptotic induction. Down-regulation of the Notch2 receptor, the pathway target Hairy/Enhancer of Split homolog 1 (Hes1), and of the stem cell markers Nestin and CD133 was also observed. Reductions in tumor mass and increases in the necrotic fraction of DAOY xenografts, in mice treated with oral ZSP were also observed, but these were not significant. CONCLUSION: ZSP can block brain tumor growth and the expression of Notch pathway members and stem cell markers in vitro.
BACKGROUND/AIM: Zeng Sheng Ping (ZSP) is a traditional herbal remedy used to prevent progression and growth of neoplastic lesions. It has been shown to inhibit Notch2 expression in a murinelung cancer model, leading us to investigate its therapeutic potential in Notch-dependent brain tumors. MATERIALS AND METHODS:3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS), apoptosis, and quantitative real-time polymerase chain reaction (RT-PCR) analyses were performed in glioma and medulloblastoma cell lines, and morphological analyses in DAOY flank xenografts. RESULTS:ZSP inhibited brain tumor growth in vitro, in part, by apoptotic induction. Down-regulation of the Notch2 receptor, the pathway target Hairy/Enhancer of Split homolog 1 (Hes1), and of the stem cell markers Nestin and CD133 was also observed. Reductions in tumor mass and increases in the necrotic fraction of DAOY xenografts, in mice treated with oral ZSP were also observed, but these were not significant. CONCLUSION:ZSP can block brain tumor growth and the expression of Notch pathway members and stem cell markers in vitro.
Authors: Karisa C Schreck; Pete Taylor; Luigi Marchionni; Vidya Gopalakrishnan; Eli E Bar; Nicholas Gaiano; Charles G Eberhart Journal: Clin Cancer Res Date: 2010-12-15 Impact factor: 12.531
Authors: Xing Fan; Leila Khaki; Thant S Zhu; Mary E Soules; Caroline E Talsma; Naheed Gul; Cheryl Koh; Jiangyang Zhang; Yue-Ming Li; Jarek Maciaczyk; Guido Nikkhah; Francesco Dimeco; Sara Piccirillo; Angelo L Vescovi; Charles G Eberhart Journal: Stem Cells Date: 2010-01 Impact factor: 6.277
Authors: Gwendolyn T Wong; Denise Manfra; Frederique M Poulet; Qi Zhang; Hubert Josien; Thomas Bara; Laura Engstrom; Maria Pinzon-Ortiz; Jay S Fine; Hu-Jung J Lee; Lili Zhang; Guy A Higgins; Eric M Parker Journal: J Biol Chem Date: 2004-01-06 Impact factor: 5.157
Authors: Zhongqiu Zhang; Yian Wang; Ruisheng Yao; Jie Li; Ying Yan; Marie La Regina; William L Lemon; Clinton J Grubbs; Ronald A Lubet; Ming You Journal: Oncogene Date: 2004-05-06 Impact factor: 9.867