PURPOSE: The aim of the study was to use micro-autoradiography to investigate the lung cell types responsible for 2-deoxy-2-[(18)F]fluoro-D-glucose (FDG) uptake in murine models of acute lung injury (ALI). PROCEDURES: C57/BL6 mice were studied in three groups: controls, ventilator-induced lung injury (VILI), and endotoxin. VILI was produced by high tidal volumes and zero end-expiratory pressure and endotoxin ALI, by intranasal administration. Following FDG injection, the lungs were processed and exposed to autoradiographic emulsion. Grain density over cells was used to quantify FDG uptake. RESULTS: Neutrophils, macrophages, and type 2 epithelial cells presented higher grain densities during VILI and endotoxin ALI than controls. Remarkably, cell grain density in specific cell types was dependent on the injury mechanism. Whereas macrophages showed high grain densities during endotoxin ALI, similar to those exhibited by neutrophils, type 2 epithelial cells demonstrated the second highest grain density (with neutrophils as the highest) during VILI. CONCLUSIONS: In murine models of VILI and endotoxin ALI, FDG uptake occurs not only in neutrophils but also in macrophages and type 2 epithelial cells. FDG uptake by individual cell types depends on the mechanism underlying ALI.
PURPOSE: The aim of the study was to use micro-autoradiography to investigate the lung cell types responsible for 2-deoxy-2-[(18)F]fluoro-D-glucose (FDG) uptake in murine models of acute lung injury (ALI). PROCEDURES: C57/BL6mice were studied in three groups: controls, ventilator-induced lung injury (VILI), and endotoxin. VILI was produced by high tidal volumes and zero end-expiratory pressure and endotoxin ALI, by intranasal administration. Following FDG injection, the lungs were processed and exposed to autoradiographic emulsion. Grain density over cells was used to quantify FDG uptake. RESULTS: Neutrophils, macrophages, and type 2 epithelial cells presented higher grain densities during VILI and endotoxin ALI than controls. Remarkably, cell grain density in specific cell types was dependent on the injury mechanism. Whereas macrophages showed high grain densities during endotoxin ALI, similar to those exhibited by neutrophils, type 2 epithelial cells demonstrated the second highest grain density (with neutrophils as the highest) during VILI. CONCLUSIONS: In murine models of VILI and endotoxin ALI, FDG uptake occurs not only in neutrophils but also in macrophages and type 2 epithelial cells. FDG uptake by individual cell types depends on the mechanism underlying ALI.
Authors: Nicolas de Prost; Eduardo L Costa; Tyler Wellman; Guido Musch; Tilo Winkler; Mauro R Tucci; R Scott Harris; Jose G Venegas; Marcos F Vidal Melo Journal: J Appl Physiol (1985) Date: 2011-07-28
Authors: V M Ranieri; P M Suter; C Tortorella; R De Tullio; J M Dayer; A Brienza; F Bruno; A S Slutsky Journal: JAMA Date: 1999-07-07 Impact factor: 56.272
Authors: Gordon D Rubenfeld; Ellen Caldwell; Eve Peabody; Jim Weaver; Diane P Martin; Margaret Neff; Eric J Stern; Leonard D Hudson Journal: N Engl J Med Date: 2005-10-20 Impact factor: 91.245
Authors: Tyler J Wellman; Tilo Winkler; Eduardo L V Costa; Guido Musch; R Scott Harris; Hui Zheng; Jose G Venegas; Marcos F Vidal Melo Journal: Crit Care Med Date: 2014-07 Impact factor: 7.598
Authors: Thomas Kiss; Thomas Bluth; Anja Braune; Robert Huhle; Axel Denz; Moritz Herzog; Johannes Herold; Luigi Vivona; Marco Millone; Alice Bergamaschi; Michael Andreeff; Martin Scharffenberg; Jakob Wittenstein; Marcos F Vidal Melo; Thea Koch; Patricia R M Rocco; Paolo Pelosi; Jörg Kotzerke; Marcelo Gama de Abreu Journal: Crit Care Med Date: 2019-04 Impact factor: 7.598
Authors: Guido Musch; Tilo Winkler; R Scott Harris; Marcos F Vidal Melo; Tyler J Wellman; Nicolas de Prost; Richard L Kradin; Jose G Venegas Journal: Anesthesiology Date: 2014-03 Impact factor: 7.892
Authors: Luciano Gattinoni; John J Marini; Antonio Pesenti; Michael Quintel; Jordi Mancebo; Laurent Brochard Journal: Intensive Care Med Date: 2016-01-18 Impact factor: 17.440
Authors: Mauro R Tucci; Eduardo L V Costa; Tyler J Wellman; Guido Musch; Tilo Winkler; R Scott Harris; Jose G Venegas; Marcelo B P Amato; Marcos F Vidal Melo Journal: Anesthesiology Date: 2013-07 Impact factor: 7.892
Authors: Tyler J Wellman; Nicolas de Prost; Mauro Tucci; Tilo Winkler; Rebecca M Baron; Piotr Filipczak; Benjamin Raby; Jen-Hwa Chu; R Scott Harris; Guido Musch; Luiz F Dos Reis Falcao; Vera Capelozzi; Jose G Venegas; Marcos F Vidal Melo Journal: Anesthesiology Date: 2016-11 Impact factor: 7.892
Authors: D L Chen; X Wang; S Yamamoto; D Carpenter; J T Engle; W Li; X Lin; D Kreisel; A S Krupnick; H J Huang; A E Gelman Journal: Am J Transplant Date: 2013-08-08 Impact factor: 8.086