| Literature DB >> 22746327 |
Hiroyasu Takemoto1, Kanjiro Miyata, Takehiko Ishii, Shota Hattori, Shigehito Osawa, Nobuhiro Nishiyama, Kazunori Kataoka.
Abstract
Herein, we report a unique technique to accelerate polymer-SNA conjugation based on copper-free click chemistry: gradual freeze-thawing of the reaction solution substantially increases the conjugation rate possibly because of the reactant concentration at the microenvironment scale. This technique was applied to the conjugation between a small interfering RNA (siRNA) and PEG in an aqueous buffer at/below room temperature.Entities:
Mesh：
Substances：
Year: 2012 PMID： 22746327 DOI： 10.1021/bc300182y
Source DB: PubMed Journal: Bioconjug Chem ISSN： 1043-1802 Impact factor: 4.774