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Literature DB >> 22744941

Bio-click chemistry: enzymatic functionalization of PEGylated capsules for targeting applications.

Melissa K M Leung¹, Christoph E Hagemeyer, Angus P R Johnston, Catalina Gonzales, Marloes M J Kamphuis, Katie Ardipradja, Georgina K Such, Karlheinz Peter, Frank Caruso.

Abstract

All sorted: The enzyme Sortase A was used to catalyze functionalization of PEGylated capsules with an activation-specific anti-platelet single-chain antibody (scFv). This enzymatic method allows fast, covalent, and site-directed functionalization of delivery vehicles under mild conditions. Activation-specific anti-platelet scFv-coated PEGylated capsules exhibited a high level of selective binding to thrombi, thus suggesting their potential for thrombosis therapy.

Entities: Disease Gene

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Year: 2012 PMID： 22744941 DOI： 10.1002/anie.201203612

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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Cited

15 in total

1. Sortase-tag expressed protein ligation: combining protein purification and site-specific bioconjugation into a single step.

Authors: Robert Warden-Rothman; Ilaria Caturegli; Vladimir Popik; Andrew Tsourkas
Journal: Anal Chem Date: 2013-10-28 Impact factor: 6.986

2. Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein.

Authors: Yizhi Qi; Miriam Amiram; Weiping Gao; Dewey G McCafferty; Ashutosh Chilkoti
Journal: Macromol Rapid Commun Date: 2013-07-09 Impact factor: 5.734

3. Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.

Authors: Christoph E Hagemeyer; Karen Alt; Angus P R Johnston; Georgina K Such; Hang T Ta; Melissa K M Leung; Sandeep Prabhu; Xiaowei Wang; Frank Caruso; Karlheinz Peter
Journal: Nat Protoc Date: 2014-12-11 Impact factor: 13.491

Bio-click chemistry: enzymatic functionalization of PEGylated capsules for targeting applications.

1. Sortase-tag expressed protein ligation: combining protein purification and site-specific bioconjugation into a single step.

2. Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein.

3. Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.

4. Targeted antithrombotic protein micelles.

Review 5. Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly.

Review 6. Site-Specific PEGylation of Therapeutic Proteins.

7. Covalent Modification of Synthetic Hydrogels with Bioactive Proteins via Sortase-Mediated Ligation.

8. A Versatile Chemo-Enzymatic Conjugation Approach Yields Homogeneous and Highly Potent Antibody-Drug Conjugates.

9. Modular Approach to the Functionalization of Polymersomes.

Review 10. Multifunctional Polymeric Nanoplatforms for Brain Diseases Diagnosis, Therapy and Theranostics.