Literature DB >> 22740262

Polydopamine-assisted immobilization of trypsin onto monolithic structures for protein digestion.

José G Rivera1, Phillip B Messersmith.   

Abstract

Inspired by the catechol-rich adhesive proteins of the mussel foot, we report a simple and versatile aqueous approach for the immobilization of trypsin onto silica and titania monolithic supports. The method involves in-situ coating of the monolithic substrates with a catechol-containing biomimetic polymer (polydopamine) derived from the polymerization of dopamine under alkaline pH, followed by conjugation of trypsin to the polydopamine polymer coating. The trypsin immobilization efficiency onto the monolithic materials was investigated as a function of different preparation parameters such as dopamine concentration and coating time. The enzymatic activity of the immobilized trypsin reactors was evaluated, and mass spectrometry based proteomic analysis was demonstrated by digestion of a model protein. The method presented in this manuscript has broad potential for immobilization of trypsin and other enzymes onto a wide variety of monolithic supports, due to the ability of polydopamine to act as a primer for covalent immobilization of proteins.
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID: 22740262     DOI: 10.1002/jssc.201200073

Source DB:  PubMed          Journal:  J Sep Sci        ISSN: 1615-9306            Impact factor:   3.645


  5 in total

1.  Poly(N-isopropylacrylamide) modified polydopamine as a temperature-responsive surface for cultivation and harvest of mesenchymal stem cells.

Authors:  Jun Zhang; Ching-An Peng
Journal:  Biomater Sci       Date:  2017-10-24       Impact factor: 6.843

2.  Chemometric optimization of trypsin digestion method applying infrared, microwave and ultrasound energies for determination of caseins and ovalbumin in wines.

Authors:  Jessy Pavón-Pérez; Karem Henriquez-Aedo; Ricardo Salazar; Miguel Herrero; Mario Aranda
Journal:  J Food Sci Technol       Date:  2020-09-18       Impact factor: 3.117

3.  Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production.

Authors:  Kanchana Dumri; Dau Hung Anh
Journal:  Enzyme Res       Date:  2014-09-10

4.  Enhancement in sustained release of antimicrobial peptide and BMP-2 from degradable three dimensional-printed PLGA scaffold for bone regeneration.

Authors:  Lei Chen; Liping Shao; Fengping Wang; Yifan Huang; Fenghui Gao
Journal:  RSC Adv       Date:  2019-04-04       Impact factor: 3.361

Review 5.  Biomedical and Clinical Importance of Mussel-Inspired Polymers and Materials.

Authors:  Nagendra Kumar Kaushik; Neha Kaushik; Sunil Pardeshi; Jai Gopal Sharma; Seung Hyun Lee; Eun Ha Choi
Journal:  Mar Drugs       Date:  2015-11-11       Impact factor: 5.118

  5 in total

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