BACKGROUND: Our recent proteomic study has shown that plasma protein levels of fetuin-B (Ft-B) and zinc-α2-glycoprotein (ZAG) are significantly elevated in obesity-resistant (OR) rats exposed to a high fat diet. Time profiling of the plasma concentrations of Ft-B and ZAG in OR rats has shown stable regulation of these proteins throughout the entire period of rat breeding. METHODS: To firmly establish roles for these proteins in lipogenesis, we efficiently knocked down (KD) the genes FETUB and AZGP1 encoding Ft-B and ZAG, respectively, using siRNA in Chang liver cells. RESULTS: Reduced expression of FETUB and AZGP1 led to a significant increase in the expression of lipogenic genes, thereby resulting in higher lipid levels in both KD cells. Collectively with our previous findings, we confirmed that Ft-B was similarly regulated with Ft-A, in that their plasma protein levels were commonly reduced in diet-induced obese rats. CONCLUSION: Our results provide a possible relationship between reduced plasma protein levels of Ft-B and ZAG and higher risk of diet-induced obesity through impaired fatty acid metabolism in hepatocytes.
BACKGROUND: Our recent proteomic study has shown that plasma protein levels of fetuin-B (Ft-B) and zinc-α2-glycoprotein (ZAG) are significantly elevated in obesity-resistant (OR) rats exposed to a high fat diet. Time profiling of the plasma concentrations of Ft-B and ZAG in OR rats has shown stable regulation of these proteins throughout the entire period of rat breeding. METHODS: To firmly establish roles for these proteins in lipogenesis, we efficiently knocked down (KD) the genes FETUB and AZGP1 encoding Ft-B and ZAG, respectively, using siRNA in Chang liver cells. RESULTS: Reduced expression of FETUB and AZGP1 led to a significant increase in the expression of lipogenic genes, thereby resulting in higher lipid levels in both KD cells. Collectively with our previous findings, we confirmed that Ft-B was similarly regulated with Ft-A, in that their plasma protein levels were commonly reduced in diet-induced obeserats. CONCLUSION: Our results provide a possible relationship between reduced plasma protein levels of Ft-B and ZAG and higher risk of diet-induced obesity through impaired fatty acid metabolism in hepatocytes.
Authors: Simara Larissa Fanalli; Bruna Pereira Martins da Silva; Julia Dezen Gomes; Vivian Vezzoni de Almeida; Felipe André Oliveira Freitas; Gabriel Costa Monteiro Moreira; Bárbara Silva-Vignato; Juliana Afonso; James Reecy; James Koltes; Dawn Koltes; Luciana Correia de Almeida Regitano; Dorian John Garrick; Júlio Cesar de Carvalho Balieiro; Ariana Nascimento Meira; Luciana Freitas; Luiz Lehmann Coutinho; Heidge Fukumasu; Gerson Barreto Mourão; Severino Matias de Alencar; Albino Luchiari Filho; Aline Silva Mello Cesar Journal: Animals (Basel) Date: 2022-06-25 Impact factor: 3.231
Authors: Hannah Steeb; Jordan M Ramsey; Paul C Guest; Pawel Stocki; Jason D Cooper; Hassan Rahmoune; Erin Ingudomnukul; Bonnie Auyeung; Liliana Ruta; Simon Baron-Cohen; Sabine Bahn Journal: Mol Autism Date: 2014-01-27 Impact factor: 7.509
Authors: Sang Woo Kim; Jung-Won Choi; Jong Won Yun; In-Sung Chung; Ho Chan Cho; Seung-Eun Song; Seung-Soon Im; Dae-Kyu Song Journal: PLoS One Date: 2019-09-10 Impact factor: 3.240