BACKGROUND: Non-amplified femtosecond laser was used to induce multiphoton effects for corneal tissue imaging and for tissue ablation. MATERIAL AND METHODS: A non-amplified titanium-sapphire laser was coupled to a laser scanning microscope in order to examine human and porcine cornea. Tissue was subjected to imaging and lesions were created using identical optical pathways at pulse energies below 2 nJ. RESULTS: Cellular components and the extracellular matrix were selectively imaged by applying autofluorescence and second harmonic generation at submicron resolution. Intrastromal linear scanning at higher power resulted in luminescent plasma along the scanning line. Lesion width decreased with increasing tissue depth and increased with increasing laser power at the target. Light microscopy showed intact stromal tissue around the area of the lesion. CONCLUSIONS: High-resolution images as well as high precision tissue lesions were created in the cornea using low energy femtosecond laser pulses. Easy switching between tissue imaging and ablation seems to be suitable for diagnostic and therapeutic applications.
BACKGROUND: Non-amplified femtosecond laser was used to induce multiphoton effects for corneal tissue imaging and for tissue ablation. MATERIAL AND METHODS: A non-amplified titanium-sapphire laser was coupled to a laser scanning microscope in order to examine human and porcine cornea. Tissue was subjected to imaging and lesions were created using identical optical pathways at pulse energies below 2 nJ. RESULTS: Cellular components and the extracellular matrix were selectively imaged by applying autofluorescence and second harmonic generation at submicron resolution. Intrastromal linear scanning at higher power resulted in luminescent plasma along the scanning line. Lesion width decreased with increasing tissue depth and increased with increasing laser power at the target. Light microscopy showed intact stromal tissue around the area of the lesion. CONCLUSIONS: High-resolution images as well as high precision tissue lesions were created in the cornea using low energy femtosecond laser pulses. Easy switching between tissue imaging and ablation seems to be suitable for diagnostic and therapeutic applications.
Authors: Warren R Zipfel; Rebecca M Williams; Richard Christie; Alexander Yu Nikitin; Bradley T Hyman; Watt W Webb Journal: Proc Natl Acad Sci U S A Date: 2003-05-19 Impact factor: 11.205
Authors: Naoyuki Morishige; Andrew J Wahlert; M Cristina Kenney; Donald J Brown; Koji Kawamoto; Tai-Ichiro Chikama; Teruo Nishida; James V Jester Journal: Invest Ophthalmol Vis Sci Date: 2007-03 Impact factor: 4.799
Authors: Naoyuki Morishige; W Matthew Petroll; Teruo Nishida; M Cristina Kenney; James V Jester Journal: J Cataract Refract Surg Date: 2006-11 Impact factor: 3.351
Authors: A Krüger; M Hovakimyan; D F Ramírez; O Stachs; H Lubatschowski; A Wree; R Guthoff; A Heisterkamp Journal: Klin Monbl Augenheilkd Date: 2009-12-15 Impact factor: 0.700