| Literature DB >> 22736013 |
Daisuke Kurita1, Akira Muto, Hyouta Himeno.
Abstract
Trans-translation is a bacterial quality control system in protein synthesis facilitated by transfer-messenger RNA (tmRNA). Here, we describe the in vitro system using purified factors to evaluate the two steps of trans-translation: peptidyl-transfer from peptidyl-tRNA to alanyl-tmRNA and decoding of the resume codon on tmRNA.Mesh：
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Year: 2012 PMID： 22736013 DOI： 10.1007/978-1-61779-949-5_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745