| Literature DB >> 22735959 |
Adam J Lowe1, Cameron L Bardliving, Carl A Batt.
Abstract
Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method for endotoxin removal at the pilot production scale using fast protein liquid chromatography and buffers specifically engineered for endotoxin removal.Mesh：
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Year: 2012 PMID： 22735959 DOI： 10.1007/978-1-61779-921-1_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745