Literature DB >> 2273255

A novel flow cytometric method for measuring protein digestion within the phagocytic vacuole of polymorphonuclear neutrophils.

A P Haynes1, J Fletcher, M Garnett, A Robins.   

Abstract

When rhodamine is attached to albumin at a high molar ratio its fluorescence is quenched but fluorescence is released when the protein is digested and the dye released. Using this observation it is possible to measure protein digestion within the phagocytic vacuole of neutrophils. The assay is simple, rapid and measures digestion even in the presence of abnormal phagocytosis.

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Year:  1990        PMID: 2273255     DOI: 10.1016/0022-1759(90)90268-z

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  2 in total

1.  Flow cytometric assay for quantifying opsonophagocytosis and killing of Staphylococcus aureus by peripheral blood leukocytes.

Authors:  E Martin; S Bhakdi
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Quantitative analysis of opsonophagocytosis and of killing of Candida albicans by human peripheral blood leukocytes by using flow cytometry.

Authors:  E Martin; S Bhakdi
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

  2 in total

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