Literature DB >> 22728344

Sensitive and robust detection of citrus greening (huanglongbing) bacterium "Candidatus Liberibacter asiaticus" by DNA amplification with new 16S rDNA-specific primers.

Takashi Fujikawa1, Toru Iwanami.   

Abstract

Citrus greening disease is caused by "Candidatus Liberibacter spp.," including "Candidatus Liberibacter asiaticus (Las)." For detecting this disease, we designed new primers from the Las 16S rDNA and used a very small DNA template for PCR. More Las-infected tissues were detected with our primers than with the common primers.
Copyright © 2012 Elsevier Ltd. All rights reserved.

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Year:  2012        PMID： 22728344     DOI： 10.1016/j.mcp.2012.06.001

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  8 in total

1.  Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples.

Authors:  Huan Chen; Ian Arthur Palmer; Jian Chen; Ming Chang; Stephen L Thompson; Fengquan Liu; Zheng Qing Fu
Journal:  J Vis Exp       Date:  2018-06-29       Impact factor: 1.355

2.  Convenient detection of the citrus greening (huanglongbing) bacterium 'Candidatus Liberibacter asiaticus' by direct PCR from the midrib extract.

Authors:  Takashi Fujikawa; Shin-Ichi Miyata; Toru Iwanami
Journal:  PLoS One       Date:  2013-02-20       Impact factor: 3.240

3.  Localization and Distribution of 'Candidatus Liberibacter asiaticus' in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody.

Authors:  Fang Ding; Yongping Duan; Cristina Paul; Ronald H Brlansky; John S Hartung
Journal:  PLoS One       Date:  2015-05-06       Impact factor: 3.240

4.  New Ca. Liberibacter psyllaurous haplotype resurrected from a 49-year-old specimen of Solanum umbelliferum: a native host of the psyllid vector.

Authors:  Kerry Elizabeth Mauck; Penglin Sun; Venkata RamaSravani Meduri; Allison K Hansen
Journal:  Sci Rep       Date:  2019-07-02       Impact factor: 4.379

5.  High efficient de novo root-to-shoot organogenesis in Citrus jambhiri Lush.: Gene expression, genetic stability and virus indexing.

Authors:  Tongbram Roshni Devi; Madhumita Dasgupta; Manas Ranjan Sahoo; Paresh Chandra Kole; Narendra Prakash
Journal:  PLoS One       Date:  2021-02-19       Impact factor: 3.240

6.  Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick.

Authors:  Luciano A Rigano; Florencia Malamud; Ingrid G Orce; Maria P Filippone; Maria R Marano; Alexandre Morais do Amaral; Atilio P Castagnaro; Adrian A Vojnov
Journal:  BMC Microbiol       Date:  2014-04-06       Impact factor: 3.605

7.  Repertoire of novel sequence signatures for the detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR.

Authors:  Sunitha Kogenaru; Qing Yan; Nadia Riera; M Caroline Roper; Xiaoling Deng; Timothy A Ebert; Michael Rogers; Michael E Irey; Gerhard Pietersen; Charles M Rush; Nian Wang
Journal:  BMC Microbiol       Date:  2014-02-17       Impact factor: 3.605

8.  Identification of the Virulence Factors of Candidatus Liberibacter asiaticus via Heterologous Expression in Nicotiana benthamiana using Tobacco Mosaic Virus.

Authors:  Xiaobao Ying; Mengyuan Wan; Linshuang Hu; Jinghua Zhang; Hui Li; Dianqiu Lv
Journal:  Int J Mol Sci       Date:  2019-11-08       Impact factor: 5.923
  8 in total

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