Literature DB >> 2272317

Development of HPLC methods for the purification and analysis of plasma membrane glycoproteins.

D Renauer1, H U Gierlich, K K Unger.   

Abstract

High resolution HPLC techniques such as affinity chromatography (AC), ion exchange chromatography (IEC), and size exclusion chromatography (SEC) were used successfully for separations of hydrophobic plasma membrane glycoproteins. We have tested a lot of commercially available columns for IEC and SEC and performed the purification of the crude plasma membrane extract with the most suitable columns. By using immobilized ligands with different specificities and sequential affinity chromatography, it is possible to obtain a preliminary structural characterization of the interesting carbohydrate residues of membrane glycoproteins.

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Year:  1990        PMID： 2272317      PMCID： PMC1568031          DOI： 10.1289/ehp.9088243

Source DB:  PubMed          Journal:  Environ Health Perspect        ISSN： 0091-6765            Impact factor:   9.031


  19 in total

1.  Separation of positional isomers of oligosaccharides and glycopeptides by high-performance anion-exchange chromatography with pulsed amperometric detection.

Authors:  M R Hardy; R R Townsend
Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

Review 2.  Fractionation and structural assessment of oligosaccharides and glycopeptides by use of immobilized lectins.

Authors:  T Osawa; T Tsuji
Journal:  Annu Rev Biochem       Date:  1987       Impact factor: 23.643

3.  Lectin-defined cell surface glycoconjugates of pancreatic cancer cells and their nonmalignant counterparts.

Authors:  A Raedler; W H Schmiegel; E Raedler; R Arndt; H G Thiele
Journal:  Exp Cell Biol       Date:  1983

4.  Enzyme-linked lectin assay (ELLA): use of alkaline phosphatase-conjugated Griffonia simplicifolia B4 isolectin for the detection of alpha-D-galactopyranosyl end groups.

Authors:  J P McCoy; J Varani; I J Goldstein
Journal:  Anal Biochem       Date:  1983-04-15       Impact factor: 3.365

Review 5.  Carbohydrates of the tumor cell surface.

Authors:  L A Smets; W P Van Beek
Journal:  Biochim Biophys Acta       Date:  1984

6.  Cell surface glycoproteins mediate compaction, trophoblast attachment, and endoderm formation during early mouse development.

Authors:  J Richa; C H Damsky; C A Buck; B B Knowles; D Solter
Journal:  Dev Biol       Date:  1985-04       Impact factor: 3.582

7.  Galactose-rich glycoproteins are on the cell surface of herpes virus-infected cells. 1. Surface labeling and serial lectin binding studies of Asn-linked oligosaccharides of glycoprotein gC.

Authors:  R Kumarasamy; H A Blough
Journal:  Arch Biochem Biophys       Date:  1985-02-01       Impact factor: 4.013

8.  Growth-inhibitory glycopeptides obtained from the cell surface of cultured chick embryo fibroblasts.

Authors:  Y Yaoi
Journal:  Exp Cell Res       Date:  1984-09       Impact factor: 3.905

9.  Separation of plasma membrane proteins of cultured human fibroblasts by affinity chromatography on bonded microparticulate silicas.

Authors:  J N Kinkel; B Anspach; K K Unger; R Wieser; G Brunner
Journal:  J Chromatogr       Date:  1984-08-03

10.  Fractionation of membrane proteins on immobilized lectins by high-performance liquid affinity chromatography.

Authors:  D Renauer; F Oesch; J Kinkel; K K Unger; R J Wieser
Journal:  Anal Biochem       Date:  1985-12       Impact factor: 3.365
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  1 in total

1.  HPLC analysis of carbohydrates on POLYSPHERCH(R)OH columns using pulsed amperometric detection (PAD) with sodium hydroxide as post column detection reagent.

Authors:  M H Gey; K K Unger; G Battermann
Journal:  Anal Bioanal Chem       Date:  1996-10       Impact factor: 4.142
  1 in total

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