OBJECTIVE: To elucidate possible mechanisms leading to neurodegeneration in patients with glucocerebrosidase (GBA)-associated Parkinson disease (PD) using combined proton ((1)H) and phosphorus ((31)P) magnetic resonance spectroscopic imaging (MRSI) in vivo. METHODS: (1)H and (1)H-decoupled (31)P MRSI was performed in 13 patients with PD with heterozygous GBA mutations (GBA-PD) and 19 age- and sex-matched healthy controls to investigate metabolite concentrations in the mesostriatal target regions of PD pathology. NAA as marker of neuronal integrity, choline and ethanolamine containing compounds as markers of membrane phospholipid metabolism, and energy metabolites (notably high-energy phosphates) were quantified. RESULTS: Compared to controls, NAA was significantly reduced in the putamen (p = 0.012) and in the midbrain of GBA-PD (p = 0.05). The choline concentration obtained from (1)H MRSI was significantly decreased in the midbrain of GBA-PD (p = 0.010). The phospholipid degradation product glycerophosphoethalonamine was increased in the putamen of GBA-PD (p = 0.05). Changes of energy metabolism were not detected in any region of interest. CONCLUSION: The pattern of neurodegeneration in GBA-associated PD is more pronounced in the putamen than in the midbrain. Our MRSI findings suggest that the neurodegenerative process in GBA-PD is associated with alterations of membrane phospholipid metabolism which might be also involved in abnormal α-synuclein aggregation.
OBJECTIVE: To elucidate possible mechanisms leading to neurodegeneration in patients with glucocerebrosidase (GBA)-associated Parkinson disease (PD) using combined proton ((1)H) and phosphorus ((31)P) magnetic resonance spectroscopic imaging (MRSI) in vivo. METHODS: (1)H and (1)H-decoupled (31)P MRSI was performed in 13 patients with PD with heterozygous GBA mutations (GBA-PD) and 19 age- and sex-matched healthy controls to investigate metabolite concentrations in the mesostriatal target regions of PD pathology. NAA as marker of neuronal integrity, choline and ethanolamine containing compounds as markers of membrane phospholipid metabolism, and energy metabolites (notably high-energy phosphates) were quantified. RESULTS: Compared to controls, NAA was significantly reduced in the putamen (p = 0.012) and in the midbrain of GBA-PD (p = 0.05). The choline concentration obtained from (1)H MRSI was significantly decreased in the midbrain of GBA-PD (p = 0.010). The phospholipid degradation product glycerophosphoethalonamine was increased in the putamen of GBA-PD (p = 0.05). Changes of energy metabolism were not detected in any region of interest. CONCLUSION: The pattern of neurodegeneration in GBA-associated PD is more pronounced in the putamen than in the midbrain. Our MRSI findings suggest that the neurodegenerative process in GBA-PD is associated with alterations of membrane phospholipid metabolism which might be also involved in abnormal α-synuclein aggregation.
Authors: Reena V Kartha; James Joers; Marcia R Terluk; Abigail Travis; Kyle Rudser; Paul J Tuite; Neal J Weinreb; Jeanine R Jarnes; James C Cloyd; Gülin Öz Journal: J Inherit Metab Dis Date: 2019-12-17 Impact factor: 4.982
Authors: Marcus Keatinge; Hai Bui; Aswin Menke; Yu-Chia Chen; Anna M Sokol; Qing Bai; Felix Ellett; Marc Da Costa; Derek Burke; Matthew Gegg; Lisa Trollope; Thomas Payne; Aimee McTighe; Heather Mortiboys; Sarah de Jager; Hugh Nuthall; Ming-Shang Kuo; Angeleen Fleming; Anthony H V Schapira; Stephen A Renshaw; J Robin Highley; Agnieszka Chacinska; Pertti Panula; Edward A Burton; Michael J O'Neill; Oliver Bandmann Journal: Hum Mol Genet Date: 2015-09-16 Impact factor: 6.150
Authors: Matthew E Gegg; Lindsay Sweet; Bing H Wang; Lamya S Shihabuddin; Sergio Pablo Sardi; Anthony H V Schapira Journal: Mov Disord Date: 2015-06-11 Impact factor: 10.338
Authors: Peter Jenner; Huw R Morris; Trevor W Robbins; Michel Goedert; John Hardy; Yoav Ben-Shlomo; Paul Bolam; David Burn; John V Hindle; David Brooks Journal: J Parkinsons Dis Date: 2013 Impact factor: 5.568