Literature DB >> 227226

ELISA for herpes simplex virus (HSV) type-specific antibodies in human sera using HSV type 1 and type 2 polyspecific antigens blocked with type-heterologous rabbit antibodies.

B F Vestergaard, P C Grauballe.   

Abstract

Crude antigenic preparations made from rabbit cornea cells infected with either HSV type 1 or type 2 could be used in ELISA for titration of HSV type-specific antibodies in human sera. After immunochemical binding of the crude HSV antigens in microtitre wells by use of type-homologous rabbit antibodies, type-common antigenic sties were blocked with type-heterologous rabbit antibodies. Titration of human sera in this system showed that high concentrations of type heterologous rabbit antibodies were capable of completely blocking type-common antigenic sites, while leaving type-specific antigenic sites unblocked and capable of reacting with human antibodies. Thus HSV type-specific antibodies in human sera could be measured in ELISA without the use of purified typespecific antigens.

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Year:  1979        PMID： 227226     DOI： 10.1111/j.1699-0463.1979.tb02436.x

Source DB:  PubMed          Journal:  Acta Pathol Microbiol Scand B        ISSN： 0105-0656


  11 in total

1.  Comparison of an enzyme-linked immunosorbent assay with indirect hemagglutination and hemagglutination inhibition for determination of rubella virus antibody: evaluation of immune status with commercial reagents in a clinical laboratory.

Authors:  A L Truant; B L Barksdale; T W Huber; L B Elliott
Journal:  J Clin Microbiol       Date:  1983-01       Impact factor: 5.948

2.  Antibody to Herpesvirus hominis in patients with carcinoma of the cervix.

Authors:  S N Najem; I G Barton; L S Al-Omar; C W Potter
Journal:  Br J Exp Pathol       Date:  1982-10

3.  Detection of rubella antibodies by hemagglutination inhibition, indirect fluorescent-antibody test, and enzyme-linked immunosorbent assay.

Authors:  M V Zartarian; G Friedly; E M Peterson; L M de la Maza
Journal:  J Clin Microbiol       Date:  1981-12       Impact factor: 5.948

4.  Isolation of the major herpes simplex virus type 1 (HSV-1)-specific glycoprotein by hydroxylapatite chromatography and its use in enzyme-linked immunosorbent assay for titration of human HSV-1-specific antibodies.

Authors:  B F Vestergaard; P C Grauballe
Journal:  J Clin Microbiol       Date:  1979-12       Impact factor: 5.948

5.  Herpes simplex virus antigen detection in human acute encephalitis: an immunohistochemical study using avidin-biotin-peroxidase complex method.

Authors:  C Charpin; D Gambarelli; M N Lavaut; J M Seigneurin; M Raphael; M Berard; M Toga
Journal:  Acta Neuropathol       Date:  1985       Impact factor: 17.088

6.  Non-linear transformation of enzyme-linked immunosorbent assay (ELISA) measurements allows usage of linear models for data analysis.

Authors:  Thomas M Lange; Maria Rotärmel; Dominik Müller; Gregory S Mahone; Friedrich Kopisch-Obuch; Harald Keunecke; Armin O Schmitt
Journal:  Virol J       Date:  2022-05-18       Impact factor: 5.913

7.  Herpes simplex virus type-selective enzyme-linked immunosorbent assay with Helix pomatia lectin-purified antigens.

Authors:  B Svennerholm; S Olofsson; S Jeansson; A Vahlne; E Lycke
Journal:  J Clin Microbiol       Date:  1984-02       Impact factor: 5.948

8.  Low level rubella immunity detected by ELISA and specific lymphocyte transformation.

Authors:  E Buimovici-Klein; A J O'Beirne; S J Millian; L Z Cooper
Journal:  Arch Virol       Date:  1980       Impact factor: 2.574

9.  Determination of herpes simplex virus type-specific antibodies by enzyme-linked immunosorbent assay.

Authors:  R M Coleman; L Pereira; P D Bailey; D Dondero; C Wickliffe; A J Nahmias
Journal:  J Clin Microbiol       Date:  1983-08       Impact factor: 5.948

10.  Prevention and control of herpesvirus diseases. Part 2. Epidemiology and immunology.

Authors: 
Journal:  Bull World Health Organ       Date:  1985       Impact factor: 9.408
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