The map-1 gene family in root-knot nematodes, Meloidogyne spp.: a set of taxonomically restricted genes specific to clonal species.

Taxonomically restricted genes (TRGs), i.e., genes that are restricted to a limited subset of phylogenetically related organisms, may be important in adaptation. In parasitic organisms, TRG-encoded proteins are possible determinants of the specificity of host-parasite interactions. In the root-knot nematode (RKN) Meloidogyne incognita, the map-1 gene family encodes expansin-like proteins that are secreted into plant tissues during parasitism, thought to act as effectors to promote successful root infection. MAP-1 proteins exhibit a modular architecture, with variable number and arrangement of 58 and 13-aa domains in their central part. Here, we address the evolutionary origins of this gene family using a combination of bioinformatics and molecular biology approaches. Map-1 genes were solely identified in one single member of the phylum Nematoda, i.e., the genus Meloidogyne, and not detected in any other nematode, thus indicating that the map-1 gene family is indeed a TRG family. A phylogenetic analysis of the distribution of map-1 genes in RKNs further showed that these genes are specifically present in species that reproduce by mitotic parthenogenesis, with the exception of M. floridensis, and could not be detected in RKNs reproducing by either meiotic parthenogenesis or amphimixis. These results highlight the divergence between mitotic and meiotic RKN species as a critical transition in the evolutionary history of these parasites. Analysis of the sequence conservation and organization of repeated domains in map-1 genes suggests that gene duplication(s) together with domain loss/duplication have contributed to the evolution of the map-1 family, and that some strong selection mechanism may be acting upon these genes to maintain their functional role(s) in the specificity of the plant-RKN interactions.

Introduction

The phylum Nematoda comprises over 25,000 described species, many of which are parasites of animals or plants [1]. Among them, root-knot nematodes (RKNs), Meloidogyne spp., are extremely polyphagous species able to infect the roots of almost all cultivated plants, being responsible for estimated losses of more than 80 billions Euros/year [2]. These nematodes are obligatory, sedentary endoparasites which have evolved an intimate interaction with their hosts, by inducing the redifferentiation of root cells into permanent feeding sites [3]. RKNs display very different modes of reproduction, from amphimixis to mitotic parthenogenesis. It is generally admitted that sexual reproduction is the ancestral state, and that parthenogenetic species evolved from amphimictic ancestors [4], [5], although a still running debate exists on whether the establishment of parthenogenesis in these nematodes is of ancient or recent origin [6], [7]. At a worldwide scale, the most agriculturally widespread and damaging species are by far the three closely related, mitotic parthenogens M. incognita, M. javanica and M. arenaria.

In M. incognita, the MAP-1 protein was originally identified as a putative avirulence factor shown to be secreted by infective juveniles [8]. It was thus hypothesized that the protein could be involved in the early steps of recognition between the plant and the nematode. At the time of its discovery, map-1 did not show any significant similarity in databases. However, a wide phylogenetic analysis recently positioned M. incognita map-1 homologues into a cluster of genes encoding expansin-like proteins [9]. Since expansins are thought to be secreted by nematodes to loosen plant cell walls when invading their hosts [10], it reinforced the hypothesis of a role of MAP-1 during the early phase(s) of the plant-nematode interaction. However, further immunolocalisation studies demonstrated that the protein was also secreted in planta by both migratory and sedentary stages, suggesting an additional role of the protein in the initiation and/or maintenance of the feeding site [11], [12]. MAP-1 exhibited highly conserved repetitive motifs of 13 and 58 aa, respectively, in its internal part [8] (Fig. 1). Recently, it was shown that map-1 belongs to a small gene family, and that variation in the number/arrangement of internal repeats in the genes was correlated with the (a)virulence of M. incognita near-isogenic lines [13]. These results clearly suggest that gene duplication, dynamic rearrangements in the repeats and gene loss are the main forces driving the evolution of the map-1 gene family in this nematode.

Figure 1

The MAP-1 protein in Meloidogyne incognita.

(A) Schematic representation of the predicted MAP-1 protein. The black arrows indicate the location of the m1/m2 and MrepF/MrepR specific primer pairs used in this study (adapted from [8]). (B) In planta immunodetection of the MAP-1 protein (green arrow) secreted by the nematode (N) in the root tissues in the viscinity of the feeding cells (FC)(adapted from [11]).

In a previous study, the genomic distribution of map-1-related sequences was found restricted to the three closely related mitotic RKN species, i.e., M. incognita, M. javanica and M. arenaria, while no homologous sequence was detected in M. hapla and M. fallax, in more distantly related plant-parasitic species (i.e., Globodera pallida, Xiphinema index) and in the free-living species Caenorhabditis elegans [8]. From that point of view, members of the map-1 gene family may be considered as ‘orphans’ or ‘taxonomically restricted genes’ (TRGs). The occurrence of such TRGs is thought to reflect important evolutionary processes, and their study could reveal the genotypic basis of exclusive ecological adaptations and the generation of lineage specific traits [14]–[16].

Recently, the genome sequences of several nematode species, including the RKNs M. incognita and M. hapla [17], [18], became available and provide new resources for in depth analysis of the evolution of the map-1 gene family. In that respect, the objective of the present study was to further investigate its phylogenetic distribution and diversity into the Nematoda phylum, using a combination of bioinformatic and PCR-based molecular approaches. Here we present evidence that members of the map-1 gene family are indeed TRGs, whose distribution is exclusively restricted to species belonging to the Meloidogyne genus. With the sole exception of M. floridensis, map-1 genes were detected further in parthenogenetic mitotic RKN species only. Sequence analysis confirmed the modular architecture of the repetitive region of these genes, and allowed the characterization of three new members of the gene family. In addition, variation was observed in the distribution of gene variants among species, with one to five genes detected per species. However, individual repeats appeared extremely conserved both intra and interspecifically in terms of their primary (nucleotide) structure, which suggests that some strong evolutionary force is acting upon them. Based on these findings, we hypothesize that gene duplications together with domain shuffling events during diversification of clonal species likely accounts for the current distribution of map-1 genes, and that these genes may be related, to some extent, to the acquisition of variable degrees of host specialization by RKNs.

Results

Phylogenetic Distribution of Map-1 Homologous Sequences is Restricted to Clonal Root-knot Nematode Species

The map-1 gene was originally identified in M. incognita, and a preliminary search among seven additional nematode taxa identified homologs in two closely related species only, i.e., M. arenaria and M. javanica [8]. To better address this issue, we have undertaken a molecular evolution study of the map-1 family using bioinformatics and molecular biology approaches. In particular, sequences corresponding to all or the repeat parts of the map-1 gene were used as queries in Blast sequence similarity searches of more extensive genomic DNA and EST databases now available in a wide range of nematode species. Under our search criteria, BlastN, BlastP and TblastX analyses against non-redundant nucleotide and/or EST databases at NCBI did not identify any discernable homologs outside of the RKN species M. arenaria, M. incognita and M. javanica. Similarly, except for the three latter species, no homolog could be identified when searching nematode-specific resources, either complete genome sequences at WormBase or extensive EST datasets at Nematode.net (Table S1). Together, results of these bioinformatic searches suggest that the taxonomic distribution of this gene family is restricted to some species of the Meloidogyne genus.

In parallel, we also developed a PCR approach to search for map-1 homologous sequences in a total of 57 nematode species belonging to 12 families and seven orders. Cloning and sequencing of the obtained amplicons confirmed the presence of map-1 homologs exclusively in RKNs, Meloidogyne spp. It is noticeable, however, that this distribution is not widespread in RKNs, but appeared to be restricted to 13 RKN species out of the 21 that were tested (Table 1). In order to infer the lineage specificity of the map-1 homologs found in this study, we replaced their distribution in the phylogenetic framework of RKNs. For that purpose, we used SSU rDNA sequences, either available in databases or generated in the laboratory (Table S2) to build a Meloidogyne tree including the 21 RKN species investigated (Figure 2). This tree is fully consistent with phylogenies previously published for the genus [19]–[21]. In particular, a clade was individualized (with 99% bootstrap support) that clustered together RKN species that reproduce by mitotic parthenogenesis, except for M. floridensis (Figure 2). Using this species tree as the foundation, we further characterized the distribution of map-1 genes among the genomes analyzed. Our results clearly showed that map-1 homologs are exclusively present in all the species belonging to the clade mentioned above. With the exception of M. floridensis, no homologous sequence was detected in meiotic parthenogenetic species nor in M. coffeicola and M. ichinohei, both of which have a mode of reproduction that remains unknown (Figure 2).

Table 1

Nematode species used in this study, with taxonomic information based on [53], [54]. Presence (+) or absence (−) of map-1 genes as inferred from PCR analysis.

Cladea	Order	Family	Species	map-1
I	Dorylaimida	Longidoridae	Xiphinema index	−
IV	Aphelenchida	Parasitaphelenchidae	Bursaphelenchus mucronatus	−
IV	Aphelenchida	Parasitaphelenchidae	Bursaphelenchus xylophilus	−
IV	Rhabditida	Steinernematidae	Steinernema sp.	−
IV	Tylenchida	Anguinidae	Ditylenchus dipsaci	−
IV	Tylenchida	Hoplolaimidae	Globodera pallida	−
IV	Tylenchida	Hoplolaimidae	Globodera rostochiensis	−
IV	Tylenchida	Hoplolaimidae	Globodera sp.	−
IV	Tylenchida	Hoplolaimidae	Globodera tabacum	−
IV	Tylenchida	Hoplolaimidae	Heterodera avenae	−
IV	Tylenchida	Hoplolaimidae	Heterodera carotae	−
IV	Tylenchida	Hoplolaimidae	Heterodera filipjevi	−
IV	Tylenchida	Hoplolaimidae	Heterodera latipons	−
IV	Tylenchida	Hoplolaimidae	Heterodera sacchari	−
IV	Tylenchida	Hoplolaimidae	Heterodera schachtii	−
IV	Tylenchida	Meloidogynidae	Meloidogyne arabicida	+
IV	Tylenchida	Meloidogynidae	Meloidogyne arenaria	+
IV	Tylenchida	Meloidogynidae	Meloidogyne artiellia	−
IV	Tylenchida	Meloidogynidae	Meloidogyne chitwoodi	−
IV	Tylenchida	Meloidogynidae	Meloidogyne coffeicola	−
IV	Tylenchida	Meloidogynidae	Meloidogyne cruciani	+
IV	Tylenchida	Meloidogynidae	Meloidogyne enterolobii	+
IV	Tylenchida	Meloidogynidae	Meloidogyne ethiopica	+
IV	Tylenchida	Meloidogynidae	Meloidogyne exigua	−
IV	Tylenchida	Meloidogynidae	Meloidogyne fallax	−
IV	Tylenchida	Meloidogynidae	Meloidogyne floridensis	+
IV	Tylenchida	Meloidogynidae	Meloidogyne hapla	−
IV	Tylenchida	Meloidogynidae	Meloidogyne hispanica	+
IV	Tylenchida	Meloidogynidae	Meloidogyne ichinohei	−
IV	Tylenchida	Meloidogynidae	Meloidogyne incognita	+
IV	Tylenchida	Meloidogynidae	Meloidogyne inornata	+
IV	Tylenchida	Meloidogynidae	Meloidogyne izalcoensis	+
IV	Tylenchida	Meloidogynidae	Meloidogyne javanica	+
IV	Tylenchida	Meloidogynidae	Meloidogyne konaensis	+
IV	Tylenchida	Meloidogynidae	Meloidogyne naasi	−
IV	Tylenchida	Meloidogynidae	Meloidogyne paranaensis	+
IV	Tylenchida	Pratylenchidae	Hirschmaniella gracilis	−
IV	Tylenchida	Pratylenchidae	Nacobbus aberrans	−
IV	Tylenchida	Pratylenchidae	Pratylenchus coffeae	−
IV	Tylenchida	Pratylenchidae	Pratylenchus crenatus	−
IV	Tylenchida	Pratylenchidae	Pratylenchus mediterraneus	−
IV	Tylenchida	Pratylenchidae	Pratylenchus neglectus	−
IV	Tylenchida	Pratylenchidae	Pratylenchus penetrans	−
IV	Tylenchida	Pratylenchidae	Pratylenchus scribneri	−
IV	Tylenchida	Pratylenchidae	Pratylenchus thornei	−
IV	Tylenchida	Pratylenchidae	Pratylenchus vulnus	−
IV	Tylenchida	Pratylenchidae	Pratylenchus zeae	−
IV	Tylenchida	Pratylenchidae	Radopholus similis	−
IV	Tylenchida	Pratylenchidae	Radopholus sp.	−
V	Rhabditida	Rhabditidae	Caenorhabditis briggsae	−
V	Rhabditida	Rhabditidae	Caenorhabditis elegans	−
V	Rhabditida	Rhabditidae	Caenorhabditis remanei	−
V	Rhabditida	Heterorhabditidae	Heterorhabditis sp.	−
V	Diplogasterida	Neodiplogasteridae	Pristionchus pacificus	−
V	Strongylida	Haemonchidae	Haemonchus contortus	−
V	Strongylida	Haemonchidae	Teladorsagia circumcincta	−
V	Strongylida	Trichostrongylidae	Trichostrongylus colubriformis	−

Clades were denominated according to [55].

Figure 2

Distribution of map-1 genes in root-knot nematodes.

A neighbor-joining phylogenetic tree of the species used in this study was built using SSU rDNA sequences shown in Table S2. Bootstrap support was calculated from 1,000 replicates and values >50% are indicated at the corresponding nodes. The red dot shows the clade clustering species that reproduce by mitotic parthenogenesis, except for M. floridensis. Red and green lines show the species that reproduce by mitotic parthenogenesis or meiotic parthenogenesis/amphimixis, respectively. Orange and blue boxes represent the 58-aa and 13-aa domains in MAP-1 encoded proteins, respectively.

Diversity of the Map-1 Gene Family in Root-knot Nematodes

By monitoring the distribution of the map-1 gene family across the Nematoda phylum, seven different members were identified in RKNs based on the architecture of the repeat region of the genes, among which four genes already described in literature [13], [22] and three new members of the family (Figure S2). Basically, the arrangement of the repeat region divided the genes into two groups, i.e., with a last short motif in 3′ orientation encoding a complete (13-aa) or a truncated (5-aa) repeat in MAP-1, respectively. Depending on the Meloidogyne species considered, one to five members of the family were detected, with a maximum number in M. izalcoensis. Interestingly, the map-1.1 gene was found present in all the 13 RKN species containing one or several map-1 homologs.

To analyze deeper the variability of the map-1 gene family in RKNs, we compared the deduced amino acid sequences resulting from the translation of the repeat region of the map-1.1 genes previously cloned in these 13 RKN species. Sequence alignments clearly showed a high level of global conservation at the amino acid level (i.e., ∼94.3%) as estimated by the ratio of conserved vs. modified positions in the 12 RKN species compared to the M. incognita MAP-1.1 sequence (Figure 3). More precisely, six substitutions and one deletion were common to the 12 RKN species, while two additionnal substitutions were shared by 11 of the 12 RKN species, respectively. Moreover, nine substitutions present in one single RKN species were distributed in four species, i.e., M. ethiopica, M. floridensis, M. izalcoensis and M. paranaensis (Figure 3).

Figure 3

Multiple alignment of the repetitive domains of the MAP-1.1 deduced protein sequences from Meloidogyne spp.

Dashes indicate deletions. Yellow boxes indicate mutated positions compared to the MAP-1.1 sequence from M. incognita.

Sequence Conservation of Map-1.1 in Meloidogyne incognita

We further evaluated the infraspecific polymorphism of the map-1 gene in M. incognita. For that purpose, a genomic fragment encompassing the map-1.1 repetitive region and its 5′ and 3′ flanking regions was amplified and directly sequenced from individual nematodes belonging to 16 M. incognita isolates from different geographic origins, either avirulent or virulent against the tomato Mi resistance gene (Table S3). Readable sequences were obtained for 122 individual nematodes, with five to ten sequences per isolate (Table S3). After trimming, these sequences could be unambiguously aligned over 591 bp, and showed a very high level of identity between them (Figure S1). Overall, compared to the original map-1.1 sequence (accession FM164760) [13], 13 positions over 591 (i.e., about 2.2%) exhibited mutations (either substitutions or deletions) and two additional single-bp insertions were observed. Variations were not equally distributed along the sequence analyzed: 1 single-bp insertion and one deletion (in 2 individuals over 122) were found in the 5′ flanking region, 5 mutations (in 9 individuals over 122) in the 3′ flanking region, while the remaining polymorphism was found concentrated in the region encoding the first 58-aa repeat (in 50 individuals over 122). Conversely, nucleotides encoding the second 58-aa repeat, and the four 13-aa repeats, appeared perfectly conserved in all the 122 individual nematodes tested (Figure S1). On the whole data set, no correlation was observed between the distribution of mutations and the (a)virulence of the isolates.

Discussion

In this study, we made use of greatly expanded sequence data sets from nematode genomes, in combination with PCR experiments, to look for homologs, in the Nematoda phylum, of the map-1 gene originally identified in the RKN species M. incognita [8]. Map-1 genes were identified in a single member of the phylum Nematoda, i.e., the genus Meloidogyne, and not detected in any other nematode, including additional species of the same clade. Moreover, the distribution of these genes appeared strictly restricted to but patchy within the Meloidoygne genus, with map-1 homologs found in 13 RKN species among the 21 experimentally tested. Based on these results, and in good agreement with very preliminary data [8], we concluded that the map-1 gene family is indeed a TRG family. Obviously, such a highly restricted pattern of distribution raises question about the origin of the map-1 gene ‘founder’, i.e., the gene that gave rise to this gene family in descendant lineages [23]. The major molecular mechanisms leading to the emergence of novel TRGs basically consist in duplication events and rearragement processes followed by fast divergence, or by de novo origination from non-coding sequences via mutations [reviewed in 24]. Alternatively, another possibility would be that map-1 was acquired by RKNs via a horizontal gene transfer (HGT) event. Such a possibility appears all the more attractive as RKNs are prone to undergo HGT, as recently demonstrated for the functional acquisition of a large repertoire of plant cell wall-degrading enzymes from bacterial origin by these nematodes [9], [25]. However, in this hypothesis, the map-1 donator organism remains unknown.

Interestingly, a more careful phylogenetic analysis of the distribution of map-1 genes in RKNs further indicated that these genes are specifically present in all the members of a sub-clade clustering together the species that reproduce by mitotic parthenogenesis, with the exception of M. floridensis. Such a congruence between the evolutionary relationship of map-1 genes and the species phylogeny is a good indication that all the genes from the family are true orthologs. The observation that map-1 was indeed detected in M. floridensis is not that surprising, since previous phylogenetic studies have shown that this species is part of the so-called clade I grouping together the mitotic RKN species, and probably results from an independent transition to meiotic parthenogenesis [19], [20], [26]. Conversely, map-1 genes could not be detected in RKNs reproducing by either meiotic parthenogenesis or amphimixis. By June 2009, there were 97 valid species in the Meloidogyne genus [27], and we conducted experiments on only 21 of them. Thus, in order to provide a more exhaustive view of map-1 representation within the genus, it will be important to examine more species as well. However, whatever its origin, we can hypothesize that the current distribution of map-1 could result either from the acquisition of the founder gene by the common ancestor of RKNs, with a subsequent loss of the gene in meiotic species after their divergence from mitotic ones, or from a later acquisition of the founder gene by the mitotic ancestor after such a divergence. Although the early or late origin of clonality in RKNs is still under debate [5]–[7], the present results highlight the divergence between mitotic and meiotic species as a critical transition in the evolutionary history of these parasites.

Three new members of the map-1 gene family have been identified in this work (i.e., genes encoding new combinations of the 58-aa and 13-aa motifs, respectively), that add up to the four members already described in M. incognita and M. javanica [8], [22], and confirm the modular architecture of the MAP-1 proteins. Many exemples of multi-domain proteins from higher eukaryotes have been described in literature [e.g., 28–31], and several molecular mechanisms are known to be involved in the creation of new gene structures including duplicated and rearranged modular domains [for review 32]. In our case, only the map-1.1 form was present in all the RKN species harbouring map-1 gene(s) in their genome, while the other forms of the genes appeared randomly distributed. To this point, and although its architecture is not the simplest that could be designed, our current hypothesis is that map-1.1 is presumably the closest to the founder gene of the entire family, and thus all the other forms of the gene are supposed to have evolved from it following duplication events. Indeed, gene duplications are considered as the primary source of new genes in nematodes [33], [34]. In the light of this hypothesis, and after initial or secondary gene duplication (or gene loss in the case of M. arabicida, M. enterolobii and M. floridensis), the observed organization of motifs within the entire set of MAP-1 proteins from closely related RKN species could result from 1) duplication or loss of one single 13-aa repeat; 2) truncation of the N-terminal 13-aa repeat; and 3) loss of both 58 and 13-aa repeats (in M. incognita, M. izalcoensis and M. paranaensis) (Figure 4). Such an evolutionary scenario at the sub-gene level involving duplication and/or loss of domains has already been documented in higher eukaryotes, for exemple in the case of snake venom metalloproteinase toxin genes [35], zinc finger genes in mammals [36] or sperm-egg-binding proteins in ancestral primates [37]. It also confirms and expand previous data obtained on M. incognita only [13]. However, considering the phylogenetic distribution of map-1 genes among RKN species, whether such evolutionary steps occurred simultaneously or independently remains to be determined.

Figure 4

Hypothetical model for the evolution of map-1 genes in root-knot nematodes.

The actual distribution of map-1 genes in Meloidogyne species is indicated at the bottom of the figure. Putative individual evolutionary events leading to such a distibution are indicated in circles according to the following code: D, L  =  gene duplication or loss, respectively; d, l, t  =  domain duplication, loss or truncation, respectively. Orange and blue boxes represent the 58-aa and 13-aa domains in MAP-1 encoded proteins, respectively.

Comparison of the repetitive region encompassing the 58 and 13-aa domains of the MAP-1.1 proteins between all 13 RKN species harbouring the corresponding gene(s) revealed a high sequence conservation. Even more remarkably, the map-1.1 genes amplified from more than one hundred individual nematodes belonging to 16 M. incognita isolates exhibited an extreme similarity in their repetitive region at the nucleotide level. Alltogether, and although further analyses are needed to confirm it definitely, this observation constitutes a reasonable indication that some purifying selection mechanism may be acting upon the gene to maintain its functional role(s). In parallel with their extensive diversity in terms of modes of reproduction, RKNs also demonstrate various degrees of specialization with respect to their host preference, with amphimictic and meiotic species having a narrow host range, while the potential host range of mitotic species encompasses the vast majority of higher plants [2], [38], [39]. Recent immunolocalisation experiments have shown that MAP-1 proteins are secreted within plant tissues during infection, whose active accumulation in the apoplasm could play a role in the recognition between the plant and the parasite [11], [12]. Do map-1 genes act to modulate conserved plant-nematode interaction mechanisms, and/or do they provide differential species-specific parasitic abilities? Resolution of these issues require to experimentally test the biochemical or phenotypic function(s) of map-1 genes, and to understand how selection works during their formation and evolution. Clearly, further investigation and understanding of these questions will continue to depend on theoretical and careful functional analyses of each individual member of the map-1 gene family.

Materials and Methods

Biological Material and Isolation of Nucleic Acids

Samples from Bursaphelenchus, Caenorhabditis, Meloidogyne and Xiphinema genera were selected from the nematode living collection at INRA, Sophia Antipolis, France. In addition, some nematodes or DNA samples were generously provided by Géraldine Anthoine, Carla De Giorgi, Godelieve Gheysen, Eric Grenier, Hans Helder, Maurice Moens, Cédric Neveu, Ralf Sommer and Myrian Tigano. Genomic DNA was prepared from ca. 200 µl of nematodes according to a standard phenol/chlorophorm protocol [40] and stored at −80°C until use. In cases where very little amounts of biological material were available, crude extracts from individual nematodes were prepared as described previously [41]. Before PCR analyses, genomic DNA samples or crude extracts from individual nematodes were diluted to 0.1–0.5 ng.µl−1 or 20 times, respectively. Quality of DNA was checked by PCR using the universal/nematode primers 988F and 1912R [42] (Table 2). In the case of RKNs, sample identity to the species level was further confirmed using either isozyme electrophoretic analyses [43] or species-specific SCAR markers [41], [44]–[46].

Table 2

Primers used in this study.

Name	Sequence (5′ to 3′)	Reference
m1	CGTGTAACAGAGATGCCAGA	[8]
m2	GTGGAGGAACAGTAAGTGAG	[8]
MRepF	GCCAGAATCATCTACACCAGGAGGACA	this study
MRepR	TGTTGTTGGAGCTGCTGGTGGTT	this study
988F	CTCAAAGATTAAGCCATGC	[42]
1912R	TTTACGGTCAGAACTAGGG	[42]
1813F	CTGCGTGAGAGGTGAAAT	[42]
2646R	GCTACCTTGTTACGACTTTT	[42]

PCR Analyses and Sequencing

Primer sequences used in this work are listed in Table 2. Reactions were performed in a final volume of 25 µl containing 200 µM dNTPs, 0.4 µM of primers, 1 U Taq DNA polymerase and 1x Taq DNA polymerase reaction buffer supplemented with MgCl2 to a final concentration of 1.5 mM according to the manufacturer’s guidelines (MP Biomedicals Taq Core kit). For the search of map-1 homologous sequences, two pairs of primers were successively used. Primers m1 and m2 amplify the central region of the map-1.2 gene encompassing the internal repeats in M. incognita, while primers MrepF and MrepR were designed at both ends of the region encoding the 58 amino-acid repeat in the MAP-1 protein (Fig. 1; Table 2). For m1/m2, cycling conditions were: 94°C for 5 min; 32x (94°C, 1 min; 58°C, 1 min; 72°C, 1 min 30 s); 72°C, 5 min. For MrepF/MrepR, cycling conditions were: 94°C for 3 min; 35x (94°C, 30 s; 54°C, 30 s; 72°C, 1 min); 72°C, 5 min. For phylogenetic analysis of RKNs, small-subunit (SSU) rDNA was amplified as two partially overlapping fragments using three universal (988F, 1813F and 2646R) and one nematode-specific (1912R) primer (Table 2) according to previously published PCR conditions [42].

Amplicons were analysed by electrophoresis in 2% agarose gels containing ethidium bromide in TBE buffer, and visualized under UV light. The selected amplicons were recovered to gel using the MiniElute Gel Extraction kit (Qiagen), ligated into a pGEM T-Easy Vector System (Promega) and transformed in Escherichia coli DH10ß competent cells. Recombinant clones were sequenced on both strands by GATC Biotech (Konstanz, Germany).

EMBL Databank Deposits

The Meloidogyne spp. sequences obtained in this work using the m1/m2 primer pair were deposited in EMBL databank under Accession Numbers HE681888-HE681910. Newly generated Meloidogyne spp. SSU rDNA sequences were deposited in EMBL databank under Accession Numbers HE667738-HE667744.

Bioinformatic Analyses

BlastN, BlastP and TBlastX search strategies [47] were conducted to identify DNA and/or protein sequences homologous to map-1 in non-redundant nucleotide, EST and protein databases at the National Center for Biotechnology Information (NCBI at http://www.ncbi.nlm.nih.gov), using sequences corresponding to all or repeat parts of the map-1.2 gene (accession AJ278663), and their theoretical amino acid sequence translations, as queries. At the nucleotide level, additional BlastN searches were performed against a set of nematode-specific databases, i.e., completed genomic sequences of 11 species at WormBase (http://www.wormbase.org) and EST clusters of 43 species at Nematode.net (http://www.nematode.net) [48]. For the complete list of the nematode species investigated, see Table 1. Putative homologs were checked for significance manually, and only sequences that aligned at least on 60% of the length of the query sequence with an E-value of 1.e−05 as the cutoff were considered as true homologs.

Nucleotide sequences were edited and translated into amino acid sequences using ExPASy online tools [49] at http://www.expasy.org. Nucleotide and amino acid sequences were aligned using MUSCLE v3.7 [50] with standard parameters at http://www.ebi.ac.uk, and alignments were refined by eye when necessary. Phylogenetic analyses were done using the Neighbor-Joining algorithm as implemented in PAUP* [51], based on mean character difference as genetic distance. Bootstrap resampling [52] with 1,000 pseudoreplicates was carried out to assess support for each individual branch.

Alignment of partial sequences amplified from 122 individuals belonging to 16 isolates. Nomenclature of isolates is as in Table S3. Stars below alignment indicate conserved positions. Orange and blue sequences above alignment encode the 58 and 13-aa repeat regions, respectively. Sequences encoding the tandemly arranged 13-aa repeats are separated by arrows. Mutated positions are highlighted in yellow.

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Distribution and structural organisation of genes in root-knot nematodes. The orange and blue boxes correspond to regions encoding the 58-aa and 13-aa repeats in MAP-1, respectively. The blue triangles indicate regions encoding the 13-aa truncated repeats.

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List of the nematode-specific nucleotide resources used in BlastN analysis. Grey shading identifies the species in which map-1 homologs were found.

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List of the rDNA SSU sequences used to infer phylogenetic relationships of root-knot nematodes.

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List of the isolates used in this study, with their geographic origin and virulence status against the tomato resistance gene.

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