AIM: To determine whether intestinal Staphylococcus spp. and their pathogenic features differed between coeliac disease (CD) patients and healthy controls. METHODS: 60 children, including active CD (n=20) and non-active CD (n=20) patients and healthy controls (n=20), were studied. Staphylococci were isolated from faeces and identified by PCR and DNA sequencing. The carriage of virulent genes, including adhesion (atlE and fbe), cell aggregation (icaD), global regulatory (agr and sar) and methicillin-resistant (mecA) genes, was analysed by PCR. RESULTS: Staphylococcus epidermidis was more abundant in the microbiota of active and non-active CD patients than in controls. Staphylococcus haemolyticus was more abundant in active CD patients than in control subjects. Staphylococcus aureus was less abundant in active CD patients than in the other child groups. Staphylococcus spp. diversity was higher in active CD patients than in non-active CD patients and controls. The presence of the mecA gene and the simultaneous presence of both the mecA and atlE genes were higher in S. epidermidis clones isolated from CD patients, with active and non-active disease, than in those from control subjects. The individual presence of the other virulent genes was lower in S. epidermidis from active CD patients than in those from the other -child- groups. CONCLUSIONS: Increased abundance of S. epidermidis carrying the mecA gene, in active and non-active CD patients, most likely reflects increased exposure of these subjects to opportunistic pathogens and antimicrobials.
AIM: To determine whether intestinal Staphylococcus spp. and their pathogenic features differed between coeliac disease (CD) patients and healthy controls. METHODS: 60 children, including active CD (n=20) and non-active CD (n=20) patients and healthy controls (n=20), were studied. Staphylococci were isolated from faeces and identified by PCR and DNA sequencing. The carriage of virulent genes, including adhesion (atlE and fbe), cell aggregation (icaD), global regulatory (agr and sar) and methicillin-resistant (mecA) genes, was analysed by PCR. RESULTS:Staphylococcus epidermidis was more abundant in the microbiota of active and non-active CDpatients than in controls. Staphylococcus haemolyticus was more abundant in active CDpatients than in control subjects. Staphylococcus aureus was less abundant in active CDpatients than in the other child groups. Staphylococcus spp. diversity was higher in active CDpatients than in non-active CDpatients and controls. The presence of the mecA gene and the simultaneous presence of both the mecA and atlE genes were higher in S. epidermidis clones isolated from CDpatients, with active and non-active disease, than in those from control subjects. The individual presence of the other virulent genes was lower in S. epidermidis from active CDpatients than in those from the other -child- groups. CONCLUSIONS: Increased abundance of S. epidermidis carrying the mecA gene, in active and non-active CDpatients, most likely reflects increased exposure of these subjects to opportunistic pathogens and antimicrobials.
Authors: Ruggiero Francavilla; Danilo Ercolini; Maria Piccolo; Lucia Vannini; Sonya Siragusa; Francesca De Filippis; Ilaria De Pasquale; Raffaella Di Cagno; Michele Di Toma; Giorgia Gozzi; Diana I Serrazanetti; Maria De Angelis; Marco Gobbetti Journal: Appl Environ Microbiol Date: 2014-03-21 Impact factor: 4.792
Authors: Aimon K Alkanani; Naoko Hara; Peter A Gottlieb; Diana Ir; Charles E Robertson; Brandie D Wagner; Daniel N Frank; Danny Zipris Journal: Diabetes Date: 2015-06-11 Impact factor: 9.461