| Literature DB >> 22717039 |
Rafael Rossetto1, Márcia Viviane Alves Saraiva, Regiane Rodrigues dos Santos, Cleidson Manoel Gomes da Silva, Luciana Rocha Faustino, Roberta Nogueira Chaves, Ivina Rocha Brito, Giovanna Quintino Rodrigues, Isadora Machado Teixeira Lima, Mariana Aragão Matos Donato, Christina Alves Peixoto, José Ricardo de Figueiredo.
Abstract
Summary This study investigated the effect of three different culture media (α minimum essential medium (α-MEM), McCoy or TCM199 during the in vitro culture (IVC) of bovine isolated pre-antral follicles. Pre-antral follicles greater than 150 μm in size were isolated and cultured for 0 (control), 8 or 16 days in one of the abovementioned culture media. Follicles were evaluated for survival, growth and antrum formation at days 8 and 16. The results showed that TCM199 was the most suitable medium to preserve follicular viability and ultrastructure, resulting in the highest rates of antrum formation. In conclusion, TCM199 promotes the in vitro development of isolated pre-antral follicles without hampering follicular functionality by sustaining in vitro growth and antrum formation.Entities:
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Year: 2012 PMID： 22717039 DOI： 10.1017/S0967199412000044
Source DB: PubMed Journal: Zygote ISSN： 0967-1994 Impact factor: 1.442