BACKGROUND: The upregulation of intercellular adhesion molecule-1 (ICAM-1) on the endothelium of blood vessels in response to pro-inflammatory stimuli is of major importance for the regulation of local inflammation in cardiovascular diseases such as atherosclerosis, myocardial infarction and stroke. In vivo molecular imaging of ICAM-1 will improve diagnosis and follow-up of patients by non-invasive monitoring of the progression of inflammation. RESULTS: A paramagnetic liposomal contrast agent functionalized with anti-ICAM-1 antibodies for multimodal magnetic resonance imaging (MRI) and fluorescence imaging of endothelial ICAM-1 expression is presented. The ICAM-1-targeted liposomes were extensively characterized in terms of size, morphology, relaxivity and the ability for binding to ICAM-1-expressing endothelial cells in vitro. ICAM-1-targeted liposomes exhibited strong binding to endothelial cells that depended on both the ICAM-1 expression level and the concentration of liposomes. The liposomes had a high longitudinal and transversal relaxivity, which enabled differentiation between basal and upregulated levels of ICAM-1 expression by MRI. The liposome affinity for ICAM-1 was preserved in the competing presence of leukocytes and under physiological flow conditions. CONCLUSION: This liposomal contrast agent displays great potential for in vivo MRI of inflammation-related ICAM-1 expression.
BACKGROUND: The upregulation of intercellular adhesion molecule-1 (ICAM-1) on the endothelium of blood vessels in response to pro-inflammatory stimuli is of major importance for the regulation of local inflammation in cardiovascular diseases such as atherosclerosis, myocardial infarction and stroke. In vivo molecular imaging of ICAM-1 will improve diagnosis and follow-up of patients by non-invasive monitoring of the progression of inflammation. RESULTS: A paramagnetic liposomal contrast agent functionalized with anti-ICAM-1 antibodies for multimodal magnetic resonance imaging (MRI) and fluorescence imaging of endothelial ICAM-1 expression is presented. The ICAM-1-targeted liposomes were extensively characterized in terms of size, morphology, relaxivity and the ability for binding to ICAM-1-expressing endothelial cells in vitro. ICAM-1-targeted liposomes exhibited strong binding to endothelial cells that depended on both the ICAM-1 expression level and the concentration of liposomes. The liposomes had a high longitudinal and transversal relaxivity, which enabled differentiation between basal and upregulated levels of ICAM-1 expression by MRI. The liposome affinity for ICAM-1 was preserved in the competing presence of leukocytes and under physiological flow conditions. CONCLUSION: This liposomal contrast agent displays great potential for in vivo MRI of inflammation-related ICAM-1 expression.
Authors: Andres J Calderon; Tridib Bhowmick; John Leferovich; Bharat Burman; Benjamin Pichette; Vladimir Muzykantov; David M Eckmann; Silvia Muro Journal: J Control Release Date: 2010-11-01 Impact factor: 9.776
Authors: Glenda S van Bochove; Leonie E M Paulis; Dolf Segers; Willem J M Mulder; Rob Krams; Klaas Nicolay; Gustav J Strijkers Journal: Contrast Media Mol Imaging Date: 2010-09-29 Impact factor: 3.161
Authors: Khosrow Khodabandehlou; Jacqueline J Masehi-Lano; Christopher Poon; Jonathan Wang; Eun Ji Chung Journal: Exp Biol Med (Maywood) Date: 2017-01-01
Authors: Lisette H Deddens; Geralda A F van Tilborg; Annette van der Toorn; Kajo van der Marel; Leonie E M Paulis; Louis van Bloois; Gert Storm; Gustav J Strijkers; Willem J M Mulder; Helga E de Vries; Rick M Dijkhuizen Journal: Mol Imaging Biol Date: 2013-08 Impact factor: 3.488