Literature DB >> 2271166

A simple procedure for maximum yield of high-quality plasmid DNA.

S Y Lee1, S Rasheed.   

Abstract

We have established a simple procedure for the rapid isolation of high-quality plasmid DNA suitable for various molecular techniques and provided a step-by-step protocol. The DNA samples isolated by this procedure have been used successfully for double-stranded DNA sequencing, restriction enzyme mapping, subcloning, in vitro mutagenesis, generation of deletion clones and so on. The procedure is highly reproducible, and superior quality DNA can be obtained without the use of phenol, chloroform or other organic solvents.

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Year:  1990        PMID： 2271166

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  74 in total

1.  Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.

Authors:  R E Parales; K Lee; S M Resnick; H Jiang; D J Lessner; D T Gibson
Journal:  J Bacteriol       Date:  2000-03       Impact factor: 3.490

2.  A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening.

Authors:  M Gunther; M Laithier; O Brison
Journal:  Mol Cell Biochem       Date:  2000-07       Impact factor: 3.396

3.  Northern, morphological, and fermentation analysis of spo0A inactivation and overexpression in Clostridium acetobutylicum ATCC 824.

Authors:  Latonia M Harris; Neil E Welker; Eleftherios T Papoutsakis
Journal:  J Bacteriol       Date:  2002-07       Impact factor: 3.490

4.  Anaerobic growth of Rhodopseudomonas palustris on 4-hydroxybenzoate is dependent on AadR, a member of the cyclic AMP receptor protein family of transcriptional regulators.

Authors:  M Dispensa; C T Thomas; M K Kim; J A Perrotta; J Gibson; C S Harwood
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

5.  Chromoplast-Targeted Proteins in Tomato (Lycopersicon esculentum Mill.) Fruit.

Authors:  S. D. Lawrence; K. Cline; G. A. Moore
Journal:  Plant Physiol       Date:  1993-07       Impact factor: 8.340

6.  Regional sequence homologies in starch-degrading enzymes.

Authors:  B J Janse; A J Steyn; I S Pretorius
Journal:  Curr Genet       Date:  1993-11       Impact factor: 3.886

7.  Identification of the pcaRKF gene cluster from Pseudomonas putida: involvement in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate.

Authors:  C S Harwood; N N Nichols; M K Kim; J L Ditty; R E Parales
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

8.  Cloning of the gene encoding peptide-binding protein 74 shows that it is a new member of the heat shock protein 70 family.

Authors:  S Z Domanico; D C DeNagel; J N Dahlseid; J M Green; S K Pierce
Journal:  Mol Cell Biol       Date:  1993-06       Impact factor: 4.272

9.  Determining the DNA sequence elements required for binding integration host factor to two different target sites.

Authors:  L M Hales; R I Gumport; J F Gardner
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

10.  Characterization of a 2,3-dihydroxybiphenyl dioxygenase from the naphthalenesulfonate-degrading bacterium strain BN6.

Authors:  G Heiss; A Stolz; A E Kuhm; C Müller; J Klein; J Altenbuchner; H J Knackmuss
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490
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