SETTING: Madang and surroundings, Papua New Guinea (PNG). OBJECTIVE: To characterise the genetic diversity and drug susceptibility of Mycobacterium tuberculosis isolates collected in Madang and surroundings. DESIGN: M. tuberculosis was isolated from sputum samples from active pulmonary tuberculosis cases. Drug resistance profiles were obtained by drug susceptibility testing. M. tuberculosis lineages were identified by single nucleotide polymorphisms and sub-typing was performed by spoligotyping. Spoligotyping and 24 locus mycobacterial interspersed repetitive units-variable number of tandem repeats were combined to identify clustered isolates. RESULTS: The 173 M. tuberculosis isolates collected belonged predominantly to the Euro-American lineage (Lineage 4) and the East-Asian lineage (Lineage 2). Multidrug-resistant M. tuberculosis were observed in 5.2% of isolates. Lineage 2 M. tuberculosis, which includes the 'Beijing' genotype, was significantly associated with any drug resistance (OR 5.2, 95%CI 1.8-15.1). Cluster analyses showed 44% molecularly clustered isolates, suggesting transmission of M. tuberculosis in the community, including transmission of primary drug-resistant M. tuberculosis. CONCLUSION: These data provide the first insight into the molecular characteristics of M. tuberculosis in the Madang area of PNG, and indicate substantial drug resistance with evidence of ongoing transmission.
SETTING: Madang and surroundings, Papua New Guinea (PNG). OBJECTIVE: To characterise the genetic diversity and drug susceptibility of Mycobacterium tuberculosis isolates collected in Madang and surroundings. DESIGN:M. tuberculosis was isolated from sputum samples from active pulmonary tuberculosis cases. Drug resistance profiles were obtained by drug susceptibility testing. M. tuberculosis lineages were identified by single nucleotide polymorphisms and sub-typing was performed by spoligotyping. Spoligotyping and 24 locus mycobacterial interspersed repetitive units-variable number of tandem repeats were combined to identify clustered isolates. RESULTS: The 173 M. tuberculosis isolates collected belonged predominantly to the Euro-American lineage (Lineage 4) and the East-Asian lineage (Lineage 2). Multidrug-resistant M. tuberculosis were observed in 5.2% of isolates. Lineage 2 M. tuberculosis, which includes the 'Beijing' genotype, was significantly associated with any drug resistance (OR 5.2, 95%CI 1.8-15.1). Cluster analyses showed 44% molecularly clustered isolates, suggesting transmission of M. tuberculosis in the community, including transmission of primary drug-resistant M. tuberculosis. CONCLUSION: These data provide the first insight into the molecular characteristics of M. tuberculosis in the Madang area of PNG, and indicate substantial drug resistance with evidence of ongoing transmission.
Authors: David Stucki; Daniela Brites; Leïla Jeljeli; Mireia Coscolla; Qingyun Liu; Andrej Trauner; Lukas Fenner; Liliana Rutaihwa; Sonia Borrell; Tao Luo; Qian Gao; Midori Kato-Maeda; Marie Ballif; Matthias Egger; Rita Macedo; Helmi Mardassi; Milagros Moreno; Griselda Tudo Vilanova; Janet Fyfe; Maria Globan; Jackson Thomas; Frances Jamieson; Jennifer L Guthrie; Adwoa Asante-Poku; Dorothy Yeboah-Manu; Eddie Wampande; Willy Ssengooba; Moses Joloba; W Henry Boom; Indira Basu; James Bower; Margarida Saraiva; Sidra E G Vaconcellos; Philip Suffys; Anastasia Koch; Robert Wilkinson; Linda Gail-Bekker; Bijaya Malla; Serej D Ley; Hans-Peter Beck; Bouke C de Jong; Kadri Toit; Elisabeth Sanchez-Padilla; Maryline Bonnet; Ana Gil-Brusola; Matthias Frank; Veronique N Penlap Beng; Kathleen Eisenach; Issam Alani; Perpetual Wangui Ndung'u; Gunturu Revathi; Florian Gehre; Suriya Akter; Francine Ntoumi; Lynsey Stewart-Isherwood; Nyanda E Ntinginya; Andrea Rachow; Michael Hoelscher; Daniela Maria Cirillo; Girts Skenders; Sven Hoffner; Daiva Bakonyte; Petras Stakenas; Roland Diel; Valeriu Crudu; Olga Moldovan; Sahal Al-Hajoj; Larissa Otero; Francesca Barletta; E Jane Carter; Lameck Diero; Philip Supply; Iñaki Comas; Stefan Niemann; Sebastien Gagneux Journal: Nat Genet Date: 2016-10-31 Impact factor: 38.330
Authors: Paul Aia; Margaret Kal; Evelyn Lavu; Lucy N John; Karen Johnson; Chris Coulter; Julia Ershova; Olga Tosas; Matteo Zignol; Shalala Ahmadova; Tauhid Islam Journal: PLoS One Date: 2016-03-22 Impact factor: 3.240