Literature DB >> 22710286

Embedding matrix for simultaneous processing of multiple histological samples.

John F Smiley1, Cynthia Bleiwas.   

Abstract

A method is described for simultaneous histological processing of multiple fixed tissue samples. The tissue samples are embedded in a gelatin-albumin protein matrix that is rapidly solidified and bound to the tissue surface using a cross-linking reagent. After freezing and sectioning, the individual sections containing multiple samples can be processed for immunocytochemical and histochemical staining. The method is demonstrated for simultaneous processing of multiple rodent brains, and for reconstruction of fragmented human postmortem brain samples.
Copyright © 2012 Elsevier B.V. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22710286      PMCID： PMC3619222          DOI： 10.1016/j.jneumeth.2012.06.005

Source DB:  PubMed          Journal:  J Neurosci Methods        ISSN： 0165-0270            Impact factor:   2.390


  5 in total

1.  Egg albumin embedding: a procedure compatible with neurological staining techniques.

Authors:  A B SNODGRESS; C H DORSEY
Journal:  Stain Technol       Date:  1963-05

2.  A rapid, sensitive histochemical stain for myelin in frozen brain sections.

Authors:  L C Schmued
Journal:  J Histochem Cytochem       Date:  1990-05       Impact factor: 2.479

3.  An improved method for embedding brain tissue in albumin-gelatin.

Authors:  A M Crane; P S Goldman
Journal:  Stain Technol       Date:  1979-03

4.  A novel immunohistochemical method for evaluation of antibody specificity and detection of labile targets in biological tissue.

Authors:  Michael Levin
Journal:  J Biochem Biophys Methods       Date:  2004-01-30

5.  Tissue microarrays for high-throughput molecular profiling of tumor specimens.

Authors:  J Kononen; L Bubendorf; A Kallioniemi; M Bärlund; P Schraml; S Leighton; J Torhorst; M J Mihatsch; G Sauter; O P Kallioniemi
Journal:  Nat Med       Date:  1998-07       Impact factor: 53.440
  5 in total
  8 in total

1.  Effects of neonatal ethanol on cerebral cortex development through adolescence.

Authors:  John F Smiley; Cynthia Bleiwas; Kurt Masiello; Eva Petkova; Judith Betz; Maria Hui; Donald A Wilson; Mariko Saito
Journal:  Brain Struct Funct       Date:  2019-05-02       Impact factor: 3.270

2.  Reduced GABA neuron density in auditory cerebral cortex of subjects with major depressive disorder.

Authors:  John F Smiley; Troy A Hackett; Cynthia Bleiwas; Eva Petkova; Aleksandar Stankov; J John Mann; Gorazd Rosoklija; Andrew J Dwork
Journal:  J Chem Neuroanat       Date:  2015-12-12       Impact factor: 3.052

3.  Selective reduction of cerebral cortex GABA neurons in a late gestation model of fetal alcohol spectrum disorder.

Authors:  John F Smiley; Mariko Saito; Cynthia Bleiwas; Kurt Masiello; Babak Ardekani; David N Guilfoyle; Scott Gerum; Donald A Wilson; Csaba Vadasz
Journal:  Alcohol       Date:  2015-07-21       Impact factor: 2.405

4.  An improved approach to align and embed multiple brain samples in a gelatin-based matrix for simultaneous histological processing.

Authors:  Kumi Nagamoto-Combs; Gunjan D Manocha; Kendra Puig; Colin K Combs
Journal:  J Neurosci Methods       Date:  2015-12-29       Impact factor: 2.390

5.  Septo-temporal distribution and lineage progression of hippocampal neurogenesis in a primate (Callithrix jacchus) in comparison to mice.

Authors:  Irmgard Amrein; Michael Nosswitz; Lutz Slomianka; R Maarten van Dijk; Stefanie Engler; Fabienne Klaus; Olivier Raineteau; Kasum Azim
Journal:  Front Neuroanat       Date:  2015-06-29       Impact factor: 3.856

6.  Effects of Strain and Species on the Septo-Temporal Distribution of Adult Neurogenesis in Rodents.

Authors:  Franziska Wiget; R Maarten van Dijk; Estelle R Louet; Lutz Slomianka; Irmgard Amrein
Journal:  Front Neurosci       Date:  2017-12-19       Impact factor: 4.677

7.  Aging Does Not Affect Axon Initial Segment Structure and Somatic Localization of Tau Protein in Hippocampal Neurons of Fischer 344 Rats.

Authors:  Andrew Kneynsberg; Nicholas M Kanaan
Journal:  eNeuro       Date:  2017-07-21

8.  Nanomechanical and Morphological AFM Mapping of Normal Tissues and Tumors on Live Brain Slices Using Specially Designed Embedding Matrix and Laser-Shaped Cantilevers.

Authors:  Vladislav M Farniev; Mikhail E Shmelev; Nikita A Shved; Valeriia S Gulaia; Arthur R Biktimirov; Alexey Y Zhizhchenko; Aleksandr A Kuchmizhak; Vadim V Kumeiko
Journal:  Biomedicines       Date:  2022-07-19
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.