| Literature DB >> 22704813 |
Dileep Kumar Kannoujia1, Saroj Kumar, Pradip Nahar.
Abstract
Herein, a simple and rapid method is described for detection of L-ascorbic acid by ascorbate oxidase immobilized onto polycarbonate strip pre-activated by 1-fluoro-2-nitro-4-azidobenzene in photochemical reaction. Covalent attachment of ascorbate oxidase was confirmed by XPS studies. The immobilized-ascorbate oxidase shows higher pH, thermal and storage stability in comparison to free enzyme.Entities:
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Year: 2012 PMID： 22704813 DOI： 10.1016/j.jbiosc.2012.05.019
Source DB: PubMed Journal: J Biosci Bioeng ISSN： 1347-4421 Impact factor: 2.894