Literature DB >> 2269446

Preparation of protoplasts and regeneration of intact cells of Streptomyces cinnamonensis.

Z Jandová1, P Tichý.   

Abstract

Protoplasts were prepared and intact cells were regenerated in Streptomyces cinnamonensis--a monensin producer--to make genetic manipulations with this strain possible. 70-80% of protoplasts were formed and up to 90% of them could regenerate into intact cells.

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Year:  1990        PMID： 2269446     DOI： 10.1007/bf02821416

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  6 in total

1.  An improved method for the preparation of Streptomycetes and Micromonospora protoplasts.

Authors:  H Ogawa; S Imai; A Satoh; M Kojima
Journal:  J Antibiot (Tokyo)       Date:  1983-02       Impact factor: 2.649

2.  Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study.

Authors:  M Okanishi; K Suzuki; H Umezawa
Journal:  J Gen Microbiol       Date:  1974-02

3.  Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration.

Authors:  R H Baltz; P Matsushima
Journal:  J Gen Microbiol       Date:  1981-11

4.  New antibiotic-producing streptomycetes, selected by antibiotic resistance as a marker. I. New antibiotic production generated by protoplast fusion treatment between Streptomyces griseus and S. tenjimariensis.

Authors:  F Yamashita; K Hotta; S Kurasawa; Y Okami; H Umezawa
Journal:  J Antibiot (Tokyo)       Date:  1985-01       Impact factor: 2.649

5.  Transformation of Streptomyces erythraeus.

Authors:  H Yamamoto; K H Maurer; C R Hutchinson
Journal:  J Antibiot (Tokyo)       Date:  1986-09       Impact factor: 2.649

6.  A comparative study of protoplast preparation and regeneration in Streptomyces rimosus and Streptomyces lividans.

Authors:  D Hranueli; T Smokvina; M Alacević
Journal:  Microbiologica       Date:  1986-07
  6 in total
  2 in total

1.  Transformation of Streptomyces lincolnensis protoplasts with plasmid vectors.

Authors:  Z Jandová; P Tichý
Journal:  Folia Microbiol (Praha)       Date:  1992       Impact factor: 2.099

2.  Properties of Saccharopolyspora erythraea strains after protoplast regeneration.

Authors:  A Rajnisz; J Solecka; W Kurzatkowski
Journal:  Folia Microbiol (Praha)       Date:  2005       Impact factor: 2.629
  2 in total

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