| Literature DB >> 2269291 |
D M Glerum1, D Claeys, W Mertens, A Azzi.
Abstract
The tricarboxylate carrier from rat liver mitochondria has been purified and reconstituted into phospholipid vesicles. Its activity has been characterized by both a radioactive citrate uptake assay and a coupled enzymatic assay. A Km of 40 microM and a Vmax of 1.56 mumol x min-1 x mg-1 have been determined for the carrier. Cholesterol levels of between 5-10% of total lipid content are shown to cause a decrease in carrier activity.Entities:
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Year: 1990 PMID： 2269291 DOI： 10.1111/j.1432-1033.1990.tb15668.x
Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956