Literature DB >> 22692733

Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples?

Enrico Tortoli1, Pasquale Urbano, Fiorella Marcelli, Tullia M Simonetti, Daniela M Cirillo.   

Abstract

Cobas Amplicor MTB and later Cobas TaqMan MTB were used to test a very large series of consecutive specimens received for tuberculosis diagnosis. Performance parameters were estimated and compared overall and for separate specimen categories. Both systems showed excellent specificity, and that of TaqMan was the higher. The sensitivities were similar but satisfactory only with respiratory specimens and smear-positive samples.

Entities:  

Mesh：

Year:  2012        PMID： 22692733      PMCID： PMC3421539          DOI： 10.1128/JCM.01412-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Multicenter study of a commercial, automated polymerase chain reaction system for the rapid detection of Mycobacterium tuberculosis in respiratory specimens in routine clinical practice.

Authors:  M Bogard; J Vincelette; R Antinozzi; R Alonso; T Fenner; J Schirm; D Aubert; C Gaudreau; E Sala; M J Ruiz-Serrano; H Petersen; L A Oostendorp; H Burkardt
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2001-10       Impact factor: 3.267

2.  Clinical evaluation of the automated COBAS AMPLICOR MTB assay for testing respiratory and nonrespiratory specimens.

Authors:  U Reischl; N Lehn; H Wolf; L Naumann
Journal:  J Clin Microbiol       Date:  1998-10       Impact factor: 5.948

3.  Clinical validation of Xpert MTB/RIF for the diagnosis of extrapulmonary tuberculosis.

Authors:  Enrico Tortoli; Cristina Russo; Claudio Piersimoni; Ester Mazzola; Paola Dal Monte; Michela Pascarella; Emanuele Borroni; Alessandra Mondo; Federica Piana; Claudio Scarparo; Luana Coltella; Giulia Lombardi; Daniela M Cirillo
Journal:  Eur Respir J       Date:  2012-01-12       Impact factor: 16.671

4.  Comparison of two automated DNA amplification systems with a manual one-tube nested PCR assay for diagnosis of pulmonary tuberculosis.

Authors:  K Y Yuen; W C Yam; L P Wong; W H Seto
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

5.  Detection of Mycobacterium tuberculosis complex in sputum specimens by the automated Roche Cobas Amplicor Mycobacterium Tuberculosis Test.

Authors:  I Rajalahti; P Vuorinen; M M Nieminen; A Miettinen
Journal:  J Clin Microbiol       Date:  1998-04       Impact factor: 5.948

6.  Evaluation of an automatic polymerase chain reaction assay for identification of Mycobacterium tuberculosis in respiratory specimens.

Authors:  I S Jan; P R Hsueh; L J Teng; L N Lee; P C Yang; K T Luh
Journal:  J Formos Med Assoc       Date:  1998-03       Impact factor: 3.282

7.  Detection of Mycobacterium tuberculosis by PCR amplification with pan-Mycobacterium primers and hybridization to an M. tuberculosis-specific probe.

Authors:  V J Tevere; P L Hewitt; A Dare; P Hocknell; A Keen; J P Spadoro; K K Young
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

8.  Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis.

Authors:  Olga L Sarmiento; Kristen A Weigle; Janet Alexander; David J Weber; William C Miller
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

9.  Direct detection of Mycobacterium tuberculosis in clinical specimens using single-tube biotinylated nested polymerase chain reaction-enzyme linked immunoassay (PCR-ELISA).

Authors:  W C Yam; V C C Cheng; W T Hui; L N Wang; W H Seto; K Y Yuen
Journal:  Diagn Microbiol Infect Dis       Date:  2004-04       Impact factor: 2.803

10.  The Cobas Amplicor MTB test for detection of Mycobacterium tuberculosis complex from respiratory and non-respiratory clinical specimens.

Authors:  Bodil Jönsson; Malin Ridell
Journal:  Scand J Infect Dis       Date:  2003
View more
  13 in total

1.  Evaluation of the Cobas TaqMan MTB test for the detection of Mycobacterium tuberculosis complex according to acid-fast-bacillus smear grades in respiratory specimens.

Authors:  Hee Jae Huh; Won-Jung Koh; Dong Joon Song; Chang-Seok Ki; Nam Yong Lee
Journal:  J Clin Microbiol       Date:  2014-11-26       Impact factor: 5.948

2.  Evaluation of the Abbott RealTime MTB and RealTime MTB INH/RIF Assays for Direct Detection of Mycobacterium tuberculosis Complex and Resistance Markers in Respiratory and Extrapulmonary Specimens.

Authors:  Sabine Hofmann-Thiel; Nikolay Molodtsov; Uladzimir Antonenka; Harald Hoffmann
Journal:  J Clin Microbiol       Date:  2016-10-12       Impact factor: 5.948

Review 3.  Importance of differential identification of Mycobacterium tuberculosis strains for understanding differences in their prevalence, treatment efficacy, and vaccine development.

Authors:  Hansong Chae; Sung Jae Shin
Journal:  J Microbiol       Date:  2018-05-02       Impact factor: 3.422

4.  Evaluation of Cobas TaqMan MTB for direct detection of the Mycobacterium tuberculosis complex in comparison with Cobas Amplicor MTB.

Authors:  Guido V Bloemberg; Antje Voit; Claudia Ritter; Vanessa Deggim; Erik C Böttger
Journal:  J Clin Microbiol       Date:  2013-04-24       Impact factor: 5.948

5.  Detection of Mycobacterium tuberculosis in blood by use of the Xpert MTB/RIF assay.

Authors:  Padmapriya P Banada; Ranie Koshy; David Alland
Journal:  J Clin Microbiol       Date:  2013-05-15       Impact factor: 5.948

6.  Pediatric Tuberculosis in Italian Children: Epidemiological and Clinical Data from the Italian Register of Pediatric Tuberculosis.

Authors:  Luisa Galli; Laura Lancella; Chiara Tersigni; Elisabetta Venturini; Elena Chiappini; Barbara Maria Bergamini; Margherita Codifava; Cristina Venturelli; Giulia Tosetti; Caterina Marabotto; Laura Cursi; Elena Boccuzzi; Silvia Garazzino; Pier Angelo Tovo; Michele Pinon; Daniele Le Serre; Laura Castiglioni; Andrea Lo Vecchio; Alfredo Guarino; Eugenia Bruzzese; Giuseppe Losurdo; Elio Castagnola; Grazia Bossi; Gian Luigi Marseglia; Susanna Esposito; Samantha Bosis; Rita Grandolfo; Valentina Fiorito; Piero Valentini; Danilo Buonsenso; Raffaele Domenici; Marco Montesanti; Filippo Maria Salvini; Enrica Riva; Icilio Dodi; Francesca Maschio; Luisa Abbagnato; Elisa Fiumana; Chiara Fornabaio; Patrizia Ballista; Vincenzo Portelli; Gabriella Bottone; Nicola Palladino; Mariella Valenzise; Barbara Vecchi; Maria Di Gangi; Carla Lupi; Alberto Villani; Maurizio de Martino
Journal:  Int J Mol Sci       Date:  2016-06-17       Impact factor: 5.923

7.  Evaluation of Fluorotype MTB for detection of Mycobacterium tuberculosis complex DNA in clinical specimens from a low-incidence country.

Authors:  Sabine Hofmann-Thiel; Harald Hoffmann
Journal:  BMC Infect Dis       Date:  2014-02-05       Impact factor: 3.090

8.  Cutaneous tuberculosis: diagnosis, histopathology and treatment - part II.

Authors:  Josemir Belo dos Santos; Ana Roberta Figueiredo; Cláudia Elise Ferraz; Márcia Helena de Oliveira; Perla Gomes da Silva; Vanessa Lucília Sileira de Medeiros
Journal:  An Bras Dermatol       Date:  2014 Jul-Aug       Impact factor: 1.896

9.  Diagnostic Molecular Mycobacteriology in Regions With Low Tuberculosis Endemicity: Combining Real-time PCR Assays for Detection of Multiple Mycobacterial Pathogens With Line Probe Assays for Identification of Resistance Mutations.

Authors:  Vanessa Deggim-Messmer; Guido V Bloemberg; Claudia Ritter; Antje Voit; Rico Hömke; Peter M Keller; Erik C Böttger
Journal:  EBioMedicine       Date:  2016-06-14       Impact factor: 8.143

Review 10.  Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis.

Authors:  Emmanuel O Babafemi; Benny P Cherian; Lee Banting; Graham A Mills; Kandala Ngianga
Journal:  Syst Rev       Date:  2017-10-25
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.