OBJECTIVE: We standardized the RT-PCR panviral CSF and determined its applicability in detecting acute enterovirus infection in the central nervous system in children under 15 years. MATERIAL AND METHODS: RT-PCR was performed directly in CSF samples of 10 pediatric patients with suspected CNS infection and 9, with different conditions of the central nervous system. RESULTS: 80% (8/10) of RT-PCR samples were positive for enterovirus in patients with suspected CNS infection and no sample was positive in patients with different ailments. CONCLUSIONS: Since enteroviruses are among the main etiologies of pediatric encephalitis, RT-PCR could be particularly useful for rapid detection in CSF.
OBJECTIVE: We standardized the RT-PCR panviral CSF and determined its applicability in detecting acute enterovirus infection in the central nervous system in children under 15 years. MATERIAL AND METHODS: RT-PCR was performed directly in CSF samples of 10 pediatric patients with suspected CNS infection and 9, with different conditions of the central nervous system. RESULTS: 80% (8/10) of RT-PCR samples were positive for enterovirus in patients with suspected CNS infection and no sample was positive in patients with different ailments. CONCLUSIONS: Since enteroviruses are among the main etiologies of pediatric encephalitis, RT-PCR could be particularly useful for rapid detection in CSF.
Authors: Joel A Vazquez-Perez; Jose E Ramirez-Gonzalez; Yazmin Moreno-Valencia; Victor A Hernandez-Hernandez; Jose A I Romero-Espinoza; Manuel Castillejos-Lopez; Andres Hernandez; Rogelio Perez-Padilla; Lizbeth E Oropeza-Lopez; Noe Escobar-Escamilla; Maribel Gonzalez-Villa; Alejandro Alejandre-Garcia; Justino Regalado-Pineda; Patricio Santillan-Doherty; Irma Lopez-Martínez; Alberto Diaz-Quiñonez; Jorge Salas-Hernandez Journal: Influenza Other Respir Viruses Date: 2016-05 Impact factor: 4.380