AIM: To investigate the allele and genotype frequencies and associated risk of interleukin (IL)-8 -251T>A polymorphism on colorectal cancer (CRC) susceptibility risk. METHODS: Peripheral blood samples of 255 normal controls and 255 clinically and histopathologically confirmed CRC patients were genotyped for IL-8 -251T>A polymorphism employing allele-specific polymerase chain reaction. The relative association of variant allele and genotypes with CRC susceptibility risk was determined by calculating the odds ratios (ORs). Corresponding χ² tests on the CRC patients and controls were carried out and 95% confidence intervals (CIs) were determined using Fisher's exact test. The allele frequencies and its risk association were calculated using FAMHAP, haplotype association analysis software. RESULTS: On comparing the frequencies of genotypes of patients and controls, the homozygous variant AA was significantly higher in CRC patients (P = 0.002) compared to controls. Investigation on the association of the polymorphic genotypes with CRC susceptibility risk, showed that the homozygous variant IL-8 -251AA had a significantly increased risk with OR 3.600 (95% CI: 1.550-8.481, P = 0.001). In the case of allele frequencies, variant allele A of IL-8 -251 showed a significantly increased risk of CRC predisposition with OR 1.32 (95% CI: 1.03-1.69, P = 0.003). CONCLUSION: Variant allele and genotype of IL-8 (-251T>A) was significantly associated with CRC susceptibility risk and could be considered as a high-risk variant for CRC predisposition.
AIM: To investigate the allele and genotype frequencies and associated risk of interleukin (IL)-8 -251T>A polymorphism on colorectal cancer (CRC) susceptibility risk. METHODS: Peripheral blood samples of 255 normal controls and 255 clinically and histopathologically confirmed CRC patients were genotyped for IL-8 -251T>A polymorphism employing allele-specific polymerase chain reaction. The relative association of variant allele and genotypes with CRC susceptibility risk was determined by calculating the odds ratios (ORs). Corresponding χ² tests on the CRC patients and controls were carried out and 95% confidence intervals (CIs) were determined using Fisher's exact test. The allele frequencies and its risk association were calculated using FAMHAP, haplotype association analysis software. RESULTS: On comparing the frequencies of genotypes of patients and controls, the homozygous variant AA was significantly higher in CRC patients (P = 0.002) compared to controls. Investigation on the association of the polymorphic genotypes with CRC susceptibility risk, showed that the homozygous variant IL-8 -251AA had a significantly increased risk with OR 3.600 (95% CI: 1.550-8.481, P = 0.001). In the case of allele frequencies, variant allele A of IL-8 -251 showed a significantly increased risk of CRC predisposition with OR 1.32 (95% CI: 1.03-1.69, P = 0.003). CONCLUSION: Variant allele and genotype of IL-8 (-251T>A) was significantly associated with CRC susceptibility risk and could be considered as a high-risk variant for CRC predisposition.
Authors: E Vairaktaris; C Yapijakis; Z Serefoglou; S Derka; S Vassiliou; E Nkenke; A Vylliotis; J Wiltfang; D Avgoustidis; E Critselis; F W Neukam; E Patsouris Journal: Eur J Surg Oncol Date: 2006-12-14 Impact factor: 4.424
Authors: Hannah P Yang; Karen Woodson; Philip R Taylor; Pirjo Pietinen; Demetrius Albanes; Jarmo Virtamo; Joseph A Tangrea Journal: Eur J Cancer Prev Date: 2006-06 Impact factor: 2.497
Authors: Lisiane B Meira; James M Bugni; Stephanie L Green; Chung-Wei Lee; Bo Pang; Diana Borenshtein; Barry H Rickman; Arlin B Rogers; Catherine A Moroski-Erkul; Jose L McFaline; David B Schauer; Peter C Dedon; James G Fox; Leona D Samson Journal: J Clin Invest Date: 2008-07 Impact factor: 14.808
Authors: Ravindran Ankathil; Mohd Aminudin Mustapha; Ahmad Aizat Abdul Aziz; Siti Nurfatimah Mohd Shahpudin; Andee Dzulkarnaen Zakaria; Muhammad Radzi Abu Hassan; Kamarul Imran Musa Journal: Asian Pac J Cancer Prev Date: 2019-06-01