Changtian Li1, Xinxin Mao, Baojun Xu. 1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, Jilin 130118, China.
Abstract
INTRODUCTION: As a Chinese herbal medicine, Jew's ear has been known for its anti-coagulant effects. Hence it is worthwhile developing an effective technique to extract active components. OBJECTIVE: To find the optimal extraction condition and to identify the best strain to yield fungal polysaccharide with anti-coagulant activity. METHODOLOGY: Three strains of Jew's ear from Jilin Province, named as 988, DY 18 and FS 02, and three extraction techniques, namely, high intensity pulsed electric fields (HIPEF), microwave-assisted extraction method (MAEM) and ultrasonic-assisted extraction method (UAEM), were applied to optimise the extraction conditions. The crude extracts and polysaccharides were further determined for anti-coagulant activities. RESULTS: All extracts prolonged blood clotting time as compared to reagent control. The HIPEF exhibited the most remarkable effect among the three extraction techniques. The anti-coagulant activities of extracts were enhanced with increasing electric field strength when the field strength reached 24 kV/cm. CONCLUSION: Current results suggest that the HIPEF technique will be an effective method in the manufacture of bioactive natural polysaccharide.
INTRODUCTION: As a Chinese herbal medicine, Jew's ear has been known for its anti-coagulant effects. Hence it is worthwhile developing an effective technique to extract active components. OBJECTIVE: To find the optimal extraction condition and to identify the best strain to yield fungal polysaccharide with anti-coagulant activity. METHODOLOGY: Three strains of Jew's ear from Jilin Province, named as 988, DY 18 and FS 02, and three extraction techniques, namely, high intensity pulsed electric fields (HIPEF), microwave-assisted extraction method (MAEM) and ultrasonic-assisted extraction method (UAEM), were applied to optimise the extraction conditions. The crude extracts and polysaccharides were further determined for anti-coagulant activities. RESULTS: All extracts prolonged blood clotting time as compared to reagent control. The HIPEF exhibited the most remarkable effect among the three extraction techniques. The anti-coagulant activities of extracts were enhanced with increasing electric field strength when the field strength reached 24 kV/cm. CONCLUSION: Current results suggest that the HIPEF technique will be an effective method in the manufacture of bioactive natural polysaccharide.