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Literature DB >> 2268280

Structures of the asparagine-linked oligosaccharides of guinea-pig factor B of the alternative complement pathway.

T Mizuochi¹, J Hamako, K Titani, M Matsushita, H Okada.

Abstract

This paper describes the structures of the asparagine-linked oligosaccharides of two forms of guinea-pig Factor B of the alternative complement pathway with different Mr values. Oligosaccharides were quantitatively liberated from both glycoproteins by hydrazinolysis, fractionated by paper electrophoresis and Bio-Gel P-4 column chromatography, and their structures determined by sequential exoglycosidase digestions in conjunction with methylation analysis. Both glycoproteins were shown to have the same biantennary complex-type oligosaccharides but it is suggested that they contain different numbers of oligosaccharide chains.

Entities: Chemical Species

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Year: 1990 PMID： 2268280 PMCID： PMC1149734 DOI： 10.1042/bj2720533

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

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References

15 in total

1. Sialyl- and fucosyltransferases in the biosynthesis of asparaginyl-linked oligosaccharides in glycoproteins. Mutually exclusive glycosylation by beta-galactoside alpha2 goes to 6 sialyltransferase and N-acetylglucosaminide alpha1 goes to 3 fucosyltransferase.

Authors: J C Paulson; J P Prieels; L R Glasgow; R L Hill
Journal: J Biol Chem Date: 1978-08-25 Impact factor: 5.157

2. Restriction of alternative complement pathway activation by sialosylglycolipids.

Authors: N Okada; T Yasuda; H Okada
Journal: Nature Date: 1982-09-16 Impact factor: 49.962

3. Analysis of oligosaccharides by gel filtration.

Authors: K Yamashita; T Mizuochi; A Kobata
Journal: Methods Enzymol Date: 1982 Impact factor: 1.600

4. Hydrazinolysis of asparagine-linked sugar chains to produce free oligosaccharides.

Authors: S Takasaki; T Mizuochi; A Kobata
Journal: Methods Enzymol Date: 1982 Impact factor: 1.600

5. Substrate specificity of Diplococcal beta-N-acetylhexosaminidase, a useful enzyme for the structural studies of complex type asparagine-linked sugar chains.

Authors: K Yamashita; T Ohkura; H Yoshima; A Kobata
Journal: Biochem Biophys Res Commun Date: 1981-05-15 Impact factor: 3.575

Structures of the asparagine-linked oligosaccharides of guinea-pig factor B of the alternative complement pathway.

1. Sialyl- and fucosyltransferases in the biosynthesis of asparaginyl-linked oligosaccharides in glycoproteins. Mutually exclusive glycosylation by beta-galactoside alpha2 goes to 6 sialyltransferase and N-acetylglucosaminide alpha1 goes to 3 fucosyltransferase.

2. Restriction of alternative complement pathway activation by sialosylglycolipids.

3. Analysis of oligosaccharides by gel filtration.

4. Hydrazinolysis of asparagine-linked sugar chains to produce free oligosaccharides.

5. Substrate specificity of Diplococcal beta-N-acetylhexosaminidase, a useful enzyme for the structural studies of complex type asparagine-linked sugar chains.

6. Fractionation of glycopeptides by affinity column chromatography on concanavalin A-sepharose.

7. The structures of the carbohydrate moieties of bovine blood coagulation factor X.

8. Structures of the asparagine-linked sugar chains of human chorionic gonadotropin.

9. Two forms of guinea pig factor B of the alternative complement pathway with different molecular weights.

10. Studies on the structures of the carbohydrate moiety of human prothrombin.