Literature DB >> 2268263

Detection of inhibition of 5-aminoimidazole-4-carboxamide ribotide transformylase by thioinosinic acid and azathioprine by a new colorimetric assay.

T Ha1, S L Morgan, W H Vaughn, I Eto, J E Baggott.   

Abstract

The colorimetric assay for 5-aminoimidazole-4-carboxamide ribotide (AICAR) transformylase (phosphoribosylamino-imidazolecarboxamide formyltransferase; EC 2.1.2.3) has been extensively modified. The modified assay is based upon the short-term permanganate oxidation of the folate product, tetrahydrofolate (H4folate) to p-aminobenzoyl glutamate (pABG). The modified assay was used to detect the transformylase activity in crude extracts of peripheral-blood mononuclear cells (PBMCs). Azathioprine and its metabolite, thioinosinic acid (tIMP), are competitive inhibitors (with respect to AICAR) of the chicken liver transformylase and the transformylase from PBMCs of the MRL/lpr mouse, an animal model of systemic autoimmune disease. The Ki values of tIMP and azathioprine for the chicken liver enzyme are 39 +/- 4 microM and 120 +/- 10 microM, whereas the Ki values for the enzyme from PBMCs of the MRL/lpr mouse are 110 +/- 20 microM and 90 +/- 14 microM respectively. The anti-inflammatory drugs ibuprofen and naproxen are also inhibitors of the transformylase.

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Year:  1990        PMID： 2268263      PMCID： PMC1149705          DOI： 10.1042/bj2720339

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

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2.  Biosynthesis of the purines. XVIII. 5-Amino-1-ribosyl-4-imidazolecarboxamide 5'-phosphate transformylase and inosinicase.

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Journal:  J Biol Chem       Date:  1957-12       Impact factor: 5.157

3.  Adenylosuccinate AMP-lyase (Neurospora crassa).

Authors:  D O Woodward
Journal:  Methods Enzymol       Date:  1978       Impact factor: 1.600

4.  Sequence-, time- and dose-dependent synergism of methotrexate and 6-mercaptopurine in malignant human T-lymphoblasts.

Authors:  J P Bökkerink; M A Bakker; T W Hulscher; R R De Abreu; E D Schretlen; J P van Laarhoven; C H De Bruyn
Journal:  Biochem Pharmacol       Date:  1986-10-15       Impact factor: 5.858

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Journal:  J Clin Invest       Date:  1978-01       Impact factor: 14.808

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Authors:  T Maruyama; T Shiota; C L Krumdieck
Journal:  Anal Biochem       Date:  1978-01       Impact factor: 3.365

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Authors:  M R Atkinson; R K Morton; A W Murray
Journal:  Biochem J       Date:  1964-08       Impact factor: 3.857

8.  Sulphasalazine in the treatment of rheumatoid arthritis: relationship of dose and serum levels to efficacy.

Authors:  T Pullar; J A Hunter; H A Capell
Journal:  Br J Rheumatol       Date:  1985-08

9.  Analysis of 6-mercaptopurine in serum or plasma using high performance liquid chromatography.

Authors:  C E Whalen; H Tamary; M Greenberg; A Zipursky; S J Soldin
Journal:  Ther Drug Monit       Date:  1985       Impact factor: 3.681

10.  Inhibition of 5-aminoimidazole-4-carboxamide ribotide transformylase, adenosine deaminase and 5'-adenylate deaminase by polyglutamates of methotrexate and oxidized folates and by 5-aminoimidazole-4-carboxamide riboside and ribotide.

Authors:  J E Baggott; W H Vaughn; B B Hudson
Journal:  Biochem J       Date:  1986-05-15       Impact factor: 3.857
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  4 in total

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Authors:  J E Baggott; G L Johanning; K E Branham; C W Prince; S L Morgan; I Eto; W H Vaughn
Journal:  Biochem J       Date:  1995-06-15       Impact factor: 3.857

2.  Cecal Metabolomic Fingerprint of Unscathed Rats: Does It Reflect the Good Response to a Provocative Decompression?

Authors:  Anne-Virginie Desruelle; Sébastien de Maistre; Sandrine Gaillard; Simone Richard; Catherine Tardivel; Jean-Charles Martin; Jean-Eric Blatteau; Alain Boussuges; Sarah Rives; Jean-Jacques Risso; Nicolas Vallee
Journal:  Front Physiol       Date:  2022-05-17       Impact factor: 4.755

3.  Inhibition of folate-dependent enzymes by non-steroidal anti-inflammatory drugs.

Authors:  J E Baggott; S L Morgan; T Ha; W H Vaughn; R J Hine
Journal:  Biochem J       Date:  1992-02-15       Impact factor: 3.857

4.  Phagocytosis and Epithelial Cell Invasion by Crohn's Disease-Associated Adherent-Invasive Escherichia coli Are Inhibited by the Anti-inflammatory Drug 6-Mercaptopurine.

Authors:  Federica Migliore; Raffaella Macchi; Paolo Landini; Moira Paroni
Journal:  Front Microbiol       Date:  2018-05-14       Impact factor: 5.640
  4 in total

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