Literature DB >> 2266972

Expression of fatty acid-binding protein from bovine heart in Escherichia coli.

E Oudenampsen1, E M Kupsch, T Wissel, F Spener, A Lezius.   

Abstract

The coding part of the cDNA of cardiac fatty acid-binding protein (cFABP) from bovine heart was cloned into the vector pKK233-2. After induction with isopropyl-beta-D-thiogalactopyranoside cFABP was found in a soluble form in the cytosol of plasmid transformed E. coli amounting up to 5.7% of the soluble protein. cFABP was detected after SDS-polyacrylamide gelelectrophoresis and/or isoelectric focusing and Western blot by immuno-staining and was determined quantitatively by a solid phase enzyme-linked immuno sorbent assay. The cFABP produced by bacteria binds oleic acid with high affinity as shown by comigration of protein and ligand in both gelfiltration and isoelectric focusing. cFABP was purified from bacterial lysates to near homogeneity and resolved into four isoproteins.

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Year:  1990        PMID： 2266972     DOI： 10.1007/bf00231370

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  8 in total

Review 1.  On the role of fatty acid binding proteins in fatty acid transport and metabolism.

Authors:  F Spener; T Börchers; M Mukherjea
Journal:  FEBS Lett       Date:  1989-02-13       Impact factor: 4.124

2.  Subcellular distribution of cardiac fatty acid-binding protein in bovine heart muscle and quantitation with an enzyme-linked immunosorbent assay.

Authors:  T Börchers; C Unterberg; H Rüdel; H Robenek; F Spener
Journal:  Biochim Biophys Acta       Date:  1989-03-14

Review 3.  The metabolic significance of mammalian fatty-acid-binding proteins: abundant proteins in search of a function.

Authors:  D A Sweetser; R O Heuckeroth; J I Gordon
Journal:  Annu Rev Nutr       Date:  1987       Impact factor: 11.848

4.  Isolation and characterization of the fatty acid binding protein from human heart.

Authors:  C Unterberg; G Heidl; D B von Bassewitz; F Spener
Journal:  J Lipid Res       Date:  1986-12       Impact factor: 5.922

5.  Bovine fatty acid binding proteins. Isolation and characterisation of two cardiac fatty acid binding proteins that are distinct from corresponding hepatic proteins.

Authors:  G Jagschies; M Reers; C Unterberg; F Spener
Journal:  Eur J Biochem       Date:  1985-11-04

6.  Expression of rat intestinal fatty acid-binding protein in Escherichia coli. Purification and comparison of ligand binding characteristics with that of Escherichia coli-derived rat liver fatty acid-binding protein.

Authors:  J B Lowe; J C Sacchettini; M Laposata; J J McQuillan; J I Gordon
Journal:  J Biol Chem       Date:  1987-04-25       Impact factor: 5.157

7.  Expression of a mammalian fatty acid-binding protein in Escherichia coli.

Authors:  J B Lowe; A W Strauss; J I Gordon
Journal:  J Biol Chem       Date:  1984-10-25       Impact factor: 5.157

8.  Cloning of a full-length complementary DNA for fatty-acid-binding protein from bovine heart.

Authors:  S Billich; T Wissel; H Kratzin; U Hahn; B Hagenhoff; A G Lezius; F Spener
Journal:  Eur J Biochem       Date:  1988-08-15
  8 in total
  2 in total

Review 1.  Cellular fatty acid-binding proteins: current concepts and future directions.

Authors:  J F Glatz; G J van der Vusse
Journal:  Mol Cell Biochem       Date:  1990 Oct 15-Nov 8       Impact factor: 3.396

2.  Expression in Escherichia coli and characterization of the fatty-acid-binding protein from human muscle.

Authors:  R A Peeters; J M Ena; J H Veerkamp
Journal:  Biochem J       Date:  1991-09-01       Impact factor: 3.857
  2 in total

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