Huijie Liu1, Ye Wang. 1. The Key Laboratory for Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China. Liuhuijie0217@gmail.com
Abstract
OBJECTIVE: To study the alkaloid chemical constituents of Lycopodium japonicum. METHOD: Compounds were isolated and purified by such methods as silica gel column chromatography, RP-C18 reversed phase column chromatography, Sephadex LH-20 column chromatography and Waters semi-preparative liquid chromatogram, and their structures were identified based on physicochemical property and spectrum data. RESULT: Nine known alkaloid chemical constituents were isolated and identified, they were lycodoline (1), lucidioline (2), alpha-obscurine (3), lycopodine (4), lycoposerramine-L (5), lycoposerramine-M (6), 11alpha-O-acetyl-lycopodine (7), des-N-methyl-a-obscurine (8), clavolonine (9). CONCLUSION: Compounds 4-9 were obtained from L. japonicum for the first time.
OBJECTIVE: To study the alkaloid chemical constituents of Lycopodium japonicum. METHOD: Compounds were isolated and purified by such methods as silica gel column chromatography, RP-C18 reversed phase column chromatography, Sephadex LH-20 column chromatography and Waters semi-preparative liquid chromatogram, and their structures were identified based on physicochemical property and spectrum data. RESULT: Nine known alkaloid chemical constituents were isolated and identified, they were lycodoline (1), lucidioline (2), alpha-obscurine (3), lycopodine (4), lycoposerramine-L (5), lycoposerramine-M (6), 11alpha-O-acetyl-lycopodine (7), des-N-methyl-a-obscurine (8), clavolonine (9). CONCLUSION: Compounds 4-9 were obtained from L. japonicum for the first time.