OBJECTIVE: To measure the thickness of the crypts and quantify the number of goblet cells of the colonic mucosa with and without intestinal transit, relating them to exclusion time. METHODS: Sixty Wistar rats were divided into three groups of 20 animals each according to the time of the final operation for the removal of the colon, in six, 12 or 18 weeks. In each group 15 animals underwent colonic exclusion by left colon proximal colostomy and distal mucous fistula, and five underwent only laparotomy (control). The colons with and without fecal stream were removed, processed and submitted to histological sections stained with hematoxylin-eosin. The height of the colonic crypts and the number of goblet cells were measured by computerized morphometry. We used the Student t test and Kruskal-Wallis test for comparison and analysis of variance, using a significance level of 5% (p <0.05). RESULTS: The height of the crypts decreased in segments without fecal stream (p =0.0001), reducing from six to 12 weeks of exclusion (p = 0.0003), stabilizing thereafter. The number of goblet cells in the crypts was smaller in segments without transit after 12 and 18 weeks (p = 0.0001), but increased as the time of exclusion progressed (p = 0.04) CONCLUSION: The exclusion of intestinal transit decreases the thickness of the colonic crypts and the number of goblet cells in the segments without transit. There is an increased number of goblet cells in the course of time exclusion.
OBJECTIVE: To measure the thickness of the crypts and quantify the number of goblet cells of the colonic mucosa with and without intestinal transit, relating them to exclusion time. METHODS: Sixty Wistar rats were divided into three groups of 20 animals each according to the time of the final operation for the removal of the colon, in six, 12 or 18 weeks. In each group 15 animals underwent colonic exclusion by left colon proximal colostomy and distal mucous fistula, and five underwent only laparotomy (control). The colons with and without fecal stream were removed, processed and submitted to histological sections stained with hematoxylin-eosin. The height of the colonic crypts and the number of goblet cells were measured by computerized morphometry. We used the Student t test and Kruskal-Wallis test for comparison and analysis of variance, using a significance level of 5% (p <0.05). RESULTS: The height of the crypts decreased in segments without fecal stream (p =0.0001), reducing from six to 12 weeks of exclusion (p = 0.0003), stabilizing thereafter. The number of goblet cells in the crypts was smaller in segments without transit after 12 and 18 weeks (p = 0.0001), but increased as the time of exclusion progressed (p = 0.04) CONCLUSION: The exclusion of intestinal transit decreases the thickness of the colonic crypts and the number of goblet cells in the segments without transit. There is an increased number of goblet cells in the course of time exclusion.
Authors: Oscar Orlando Araya Fernandez; José Aires Pereira; Fábio Guilherme Campos; Carolina Mardegan Araya; Gabriele Escocia Marinho; Rafaela de Souza Novo; Thais Silva de Oliveira; Yara Tinoco Franceschi; Carlos Augusto Real Martinez Journal: Arq Bras Cir Dig Date: 2017 Apr-Jun
Authors: Flora Scott; Sandra G Gonzalez Malagon; Brett A O'Brien; Diede Fennema; Sunil Veeravalli; Clarissa R Coveney; Ian R Phillips; Elizabeth A Shephard Journal: Drug Metab Dispos Date: 2017-06-23 Impact factor: 3.922
Authors: Oscar Orlando Araya Fernandez; José Aires Pereira; Fábio Guilherme Campos; Carolina Mardegan Araya; Gabriele Escocia Marinho; Rafaela de Souza Novo; Thais Silva de Oliveira; Yara Tinoco Franceschi; Carlos Augusto Real Martinez Journal: Arq Bras Cir Dig Date: 2018-08-16
Authors: Tarek Hamdy Abd-Elhamid; Iman A M Abdel-Rahman; Amany Refaat Mahmoud; Khaled S Allemailem; Ahmad Almatroudi; Samer S Fouad; Osama H Abdella; Hatem A Elshabrawy; Asmaa M El-Kady Journal: Antibiotics (Basel) Date: 2021-04-21
Authors: Arthur Medeiros Lima; Carlos Eduardo Costa Nascimento; Carlos Henrique Marques Dos Santos; Doroty Mesquita Dourado; Gabriel Elias Cardoso Siqueira; Giovana Maria Rigo; Lauren Umpierre Bernardi; Paulo Otávio Souza Leonel; Rosemary Matias; Vitor Caldas Ferreira; Vitor Cruz Rosa Pires de Souza Journal: Arq Bras Cir Dig Date: 2019-12-09