Literature DB >> 22658555

Synthesis of ¹³C- and ¹⁴C-labeled dinucleotide mRNA cap analogues for structural and biochemical studies.

Karolina Piecyk1, Richard E Davis, Marzena Jankowska-Anyszka.   

Abstract

Herein we describe the first simple and short method for specific labeling of mono- and trimethylated dinucleotide mRNA cap analogues with (13)C and (14)C isotopes. The labels were introduced within the cap structures either at the N7 for monomethylguanosine cap or N7 and N2 position for trimethylguanosine cap. The compounds designed for structural and biochemical studies will be useful tools for better understanding the role of the mRNA cap structures in pre-mRNA splicing, nucleocytoplasmic transport, translation initiation and mRNA degradation.
Copyright © 2012 Elsevier Ltd. All rights reserved.

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Year:  2012        PMID： 22658555      PMCID： PMC3652009          DOI： 10.1016/j.bmcl.2012.04.120

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  23 in total

1.  Syntheses of nucleoside triphosphates.

Authors:  K Burgess; D Cook
Journal:  Chem Rev       Date:  2000-06-14       Impact factor: 60.622

2.  Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS.

Authors:  Leah S Cohen; Claudette Mikhli; Cassandra Friedman; Marzena Jankowska-Anyszka; Janusz Stepinski; Edward Darzynkiewicz; Richard E Davis
Journal:  RNA       Date:  2004-10       Impact factor: 4.942

3.  Multiple isoforms of eukaryotic protein synthesis initiation factor 4E in Caenorhabditis elegans can distinguish between mono- and trimethylated mRNA cap structures.

Authors:  M Jankowska-Anyszka; B J Lamphear; E J Aamodt; T Harrington; E Darzynkiewicz; R Stolarski; R E Rhoads
Journal:  J Biol Chem       Date:  1998-04-24       Impact factor: 5.157

4.  Structure of translation factor eIF4E bound to m7GDP and interaction with 4E-binding protein.

Authors:  H Matsuo; H Li; A M McGuire; C M Fletcher; A C Gingras; N Sonenberg; G Wagner
Journal:  Nat Struct Biol       Date:  1997-09

5.  Functional characterization of five eIF4E isoforms in Caenorhabditis elegans.

Authors:  B D Keiper; B J Lamphear; A M Deshpande; M Jankowska-Anyszka; E J Aamodt; T Blumenthal; R E Rhoads
Journal:  J Biol Chem       Date:  2000-04-07       Impact factor: 5.157

6.  Quantitative assessment of mRNA cap analogues as inhibitors of in vitro translation.

Authors:  A Cai; M Jankowska-Anyszka; A Centers; L Chlebicka; J Stepinski; R Stolarski; E Darzynkiewicz; R E Rhoads
Journal:  Biochemistry       Date:  1999-06-29       Impact factor: 3.162

7.  In vivo translation and stability of trans-spliced mRNAs in nematode embryos.

Authors:  Guofeng Cheng; Leah Cohen; Claudette Mikhli; Marzena Jankowska-Anyszka; Janusz Stepinski; Edward Darzynkiewicz; Richard E Davis
Journal:  Mol Biochem Parasitol       Date:  2007-02-21       Impact factor: 1.759

8.  Biophysical studies of eIF4E cap-binding protein: recognition of mRNA 5' cap structure and synthetic fragments of eIF4G and 4E-BP1 proteins.

Authors:  Anna Niedzwiecka; Joseph Marcotrigiano; Janusz Stepinski; Marzena Jankowska-Anyszka; Aleksandra Wyslouch-Cieszynska; Michal Dadlez; Anne-Claude Gingras; Pawel Mak; Edward Darzynkiewicz; Nahum Sonenberg; Stephen K Burley; Ryszard Stolarski
Journal:  J Mol Biol       Date:  2002-06-07       Impact factor: 5.469

9.  Structural changes of eIF4E upon binding to the mRNA 5' monomethylguanosine and trimethylguanosine Cap.

Authors:  Izabela Rutkowska-Wlodarczyk; Janusz Stepinski; Michal Dadlez; Edward Darzynkiewicz; Ryszard Stolarski; Anna Niedzwiecka
Journal:  Biochemistry       Date:  2008-01-26       Impact factor: 3.162

10.  The C. elegans trans-spliced leader RNA is bound to Sm and has a trimethylguanosine cap.

Authors:  J D Thomas; R C Conrad; T Blumenthal
Journal:  Cell       Date:  1988-08-12       Impact factor: 41.582
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  1 in total

1.  Selective Enzymatic Demethylation of N2 ,N2 -Dimethylguanosine in RNA and Its Application in High-Throughput tRNA Sequencing.

Authors:  Qing Dai; Guanqun Zheng; Michael H Schwartz; Wesley C Clark; Tao Pan
Journal:  Angew Chem Int Ed Engl       Date:  2017-03-30       Impact factor: 15.336
  1 in total

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