Literature DB >> 22652082

Simple procedure for fatty acid analysis of glycerophospholipids in Escherichia coli and Saccharomyces cerevisiae.

Ken'ichi Ichihara1, Atsumi Tani.   

Abstract

Rapid, convenient methods have been developed for fatty acid analysis of membrane glycerophospholipids in microorganisms. Fatty acid methyl esters derived from glycerophospholipids have been prepared directly from wet pellets of Escherichia coli cells or Saccharomyces cerevisiae spheroplasts without lipid extraction and fractionation in high yields under mild temperature conditions for analysis by gas chromatography.
Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

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Year:  2012        PMID: 22652082     DOI: 10.1016/j.jbiosc.2012.04.024

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN: 1347-4421            Impact factor:   2.894


  1 in total

1.  Cyclopropane fatty acid biosynthesis in plants: phylogenetic and biochemical analysis of Litchi Kennedy pathway and acyl editing cycle genes.

Authors:  Jay Shockey; David Kuhn; Tao Chen; Heping Cao; Barbara Freeman; Catherine Mason
Journal:  Plant Cell Rep       Date:  2018-08-06       Impact factor: 4.570

  1 in total

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