Literature DB >> 226515

Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay.

H Sankaran, C W Deveney, I D Goldfine, J A Williams.   

Abstract

A modified method is described for the preparation of stable, high specific activity radioiodinated cholecystokinin (CCK) by its conjugation to 125I-Bolton Hunter reagent (125I-BH). Purified radioiodinated CCK (125I-BH-CCK) stimulated amylase release from isolate rat pancreatic acini with a potency identical to that of native CCK. Further, 125I-BH-CCK was highly immunoreactive and reacted with antisera directed toward both the NH2- and COOH-terminal portions of the CCK molecule. Finally, 125I-BH-CCK bound to specific receptor sites on isolated pancreatic acini.

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Year:  1979        PMID： 226515

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  7 in total

1.  Evidence for a common evolutionary origin of brain and pancreas cholecystokinin receptors.

Authors:  S R Vigna; M C Thorndyke; J A Williams
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

2.  Distinct cholecystokinin receptors in brain and pancreas.

Authors:  R B Innis; S H Snyder
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

3.  Proglumide (gastrin and cholecystokinin receptor antagonist) inhibits insulin secretion in vitro.

Authors:  E J Verspohl; G Wunderle; H P Ammon; J A Williams; I D Goldfine
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1986-03       Impact factor: 3.000

4.  Chronic oral administration of synthetic trypsin inhibitor camostate reduces amylase release from isolated rat pancreatic acini.

Authors:  M Otsuki; M Fujii; T Nakamura; S Tani; Y Okabayashi
Journal:  Int J Pancreatol       Date:  1995-10

5.  Identification and localization of cholecystokinin-binding sites on rat pancreatic plasma membranes and acinar cells: a biochemical and autoradiographic study.

Authors:  S A Rosenzweig; L J Miller; J D Jamieson
Journal:  J Cell Biol       Date:  1983-05       Impact factor: 10.539

6.  Stimulus-secretion coupling in the developing exocrine pancreas: secretory responsiveness to cholecystokinin.

Authors:  A Chang; J D Jamieson
Journal:  J Cell Biol       Date:  1986-12       Impact factor: 10.539

7.  Analysis of cholecystokinin-binding proteins using endo-beta-N-acetylglucosaminidase F.

Authors:  S A Rosenzweig; L D Madison; J D Jamieson
Journal:  J Cell Biol       Date:  1984-09       Impact factor: 10.539
  7 in total

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