Literature DB >> 2264931

A simplified method for the preparation of transcriptionally active liver nuclear extracts.

M Hattori1, A Tugores, L Veloz, M Karin, D A Brenner.   

Abstract

We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.

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Year:  1990        PMID： 2264931     DOI： 10.1089/dna.1990.9.777

Source DB:  PubMed          Journal:  DNA Cell Biol        ISSN： 1044-5498            Impact factor:   3.311


  33 in total

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Journal:  Mol Cell Biol       Date:  1997-09       Impact factor: 4.272

2.  Transcription factors nuclear factor I and Sp1 interact with the murine collagen alpha 1 (I) promoter.

Authors:  M C Nehls; R A Rippe; L Veloz; D A Brenner
Journal:  Mol Cell Biol       Date:  1991-08       Impact factor: 4.272

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Authors:  B K Nayak; B R Das
Journal:  Mol Biol Rep       Date:  1999-12       Impact factor: 2.316

4.  Differential regulation of the retinoblastoma family of proteins during cell proliferation and differentiation.

Authors:  J Garriga; A Limón; X Mayol; S G Rane; J H Albrecht; E P Reddy; V Andrés; X Graña
Journal:  Biochem J       Date:  1998-08-01       Impact factor: 3.857

5.  NFkappaB prevents apoptosis and liver dysfunction during liver regeneration.

Authors:  Y Iimuro; T Nishiura; C Hellerbrand; K E Behrns; R Schoonhoven; J W Grisham; D A Brenner
Journal:  J Clin Invest       Date:  1998-02-15       Impact factor: 14.808

6.  Xylulose 5-phosphate mediates glucose-induced lipogenesis by xylulose 5-phosphate-activated protein phosphatase in rat liver.

Authors:  Tsutomu Kabashima; Takumi Kawaguchi; Brian E Wadzinski; Kosaku Uyeda
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-08       Impact factor: 11.205

7.  After portal branch ligation in the rat, cellular proliferation is associated with selective induction of c-Ha-ras, p53, cyclin E, and Cdk2.

Authors:  P Stärkel; L Lambotte; C Sempoux; C De Saeger; A Saliez; D Maiter; Y Horsmans
Journal:  Gut       Date:  2001-07       Impact factor: 23.059

8.  A glucose-responsive transcription factor that regulates carbohydrate metabolism in the liver.

Authors:  H Yamashita; M Takenoshita; M Sakurai; R K Bruick; W J Henzel; W Shillinglaw; D Arnot; K Uyeda
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-24       Impact factor: 11.205

9.  Identification of a nuclear protein interacting with a novel site on rat androgen receptor promoter after transcription factor NFkB is displaced from adjacent site.

Authors:  Ghazala Zaidi; Prakash C Supakar
Journal:  Mol Biol Rep       Date:  2003-06       Impact factor: 2.316

10.  Early overexpression of Cdk4 and possible role of KRF and c-myc in chewing tobacco mediated oral cancer development.

Authors:  Rajakishore Mishra; Bibhu Ranjan Das
Journal:  Mol Biol Rep       Date:  2003-12       Impact factor: 2.316
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