Literature DB >> 2264833

Maintenance of bile acid synthesis and cholesterol 7 alpha-hydroxylase activity in cultured rat hepatocytes.

H M Princen1, P Meijer.   

Abstract

Addition of foetal-bovine serum to rat hepatocytes cultured in Williams E medium resulted in improved maintenance of bile-acid-synthetic capacity and cholesterol 7 alpha-hydroxylase activity as compared with cultures supplemented with rat or newborn-bovine serum or cultures in a hormonally defined serum-free medium. Minimally, 5% (v/v) foetal-bovine serum was necessary to maintain these liver-specific functions. Serum factor(s) responsible for these effects were not dialysable or associated with lipoproteins, but were removed by charcoal extraction.

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Year:  1990        PMID： 2264833      PMCID： PMC1149691          DOI： 10.1042/bj2720273

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  21 in total

1.  Bile acid synthesis and secretion by rabbit hepatocytes in primary monolayer culture: comparison with rat hepatocytes.

Authors:  M J Whiting; R A Wishart; M R Gowing; M E McManus; A M Mackinnon
Journal:  Biochim Biophys Acta       Date:  1989-02-06

2.  Assay of cholesterol 7 alpha-hydroxylase activity in rat hepatocytes in primary monolayer culture.

Authors:  H M Princen; P Meijer; J Kwekkeboom; H J Kempen
Journal:  Anal Biochem       Date:  1988-05-15       Impact factor: 3.365

3.  Secretion of the newly synthesized cholesterol by rat hepatocytes in primary culture.

Authors:  R C Lin
Journal:  Biochim Biophys Acta       Date:  1984-04-18

4.  Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix.

Authors:  R Enat; D M Jefferson; N Ruiz-Opazo; Z Gatmaitan; L A Leinwand; L M Reid
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

5.  Stimulation of the LDL receptor activity in the human hepatoma cell line Hep G2 by high-density serum fractions.

Authors:  L M Havekes; D Schouten; E C de Wit; L H Cohen; M Griffioen; V W van Hinsbergh; H M Princen
Journal:  Biochim Biophys Acta       Date:  1986-02-12

6.  Ketoconazole blocks bile acid synthesis in hepatocyte monolayer cultures and in vivo in rat by inhibiting cholesterol 7 alpha-hydroxylase.

Authors:  H M Princen; C M Huijsmans; F Kuipers; R J Vonk; H J Kempen
Journal:  J Clin Invest       Date:  1986-10       Impact factor: 14.808

7.  Studies on the maintenance of cytochromes P-450 and b5, monooxygenases and cytochrome reductases in primary cultures of rat hepatocytes.

Authors:  M H Grant; M A Melvin; P Shaw; W T Melvin; M D Burke
Journal:  FEBS Lett       Date:  1985-10-07       Impact factor: 4.124

8.  Characterisation of rat hepatocyte monolayers for investigation of the metabolism of bile salts.

Authors:  R P Ford; K M Botham; K E Suckling; G S Boyd
Journal:  Biochim Biophys Acta       Date:  1985-09-11

9.  Changes in the concentration of seven forms of cytochrome P-450 in primary cultures of adult rat hepatocytes.

Authors:  A R Steward; G A Dannan; P S Guzelian; F P Guengerich
Journal:  Mol Pharmacol       Date:  1985-01       Impact factor: 4.436

10.  Suitability of primary monolayer cultures of adult rat hepatocytes for studies of cholesterol and bile acid metabolism.

Authors:  P B Hylemon; E C Gurley; W M Kubaska; T R Whitehead; P S Guzelian; Z R Vlahcevic
Journal:  J Biol Chem       Date:  1985-01-25       Impact factor: 5.157
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  3 in total

Review 1.  Review of progress in sterol oxidations: 1987-1995.

Authors:  L L Smith
Journal:  Lipids       Date:  1996-05       Impact factor: 1.880

2.  Differential feedback regulation of cholesterol 7 alpha-hydroxylase mRNA and transcriptional activity by rat bile acids in primary monolayer cultures of rat hepatocytes.

Authors:  J Twisk; E M Lehmann; H M Princen
Journal:  Biochem J       Date:  1993-03-15       Impact factor: 3.857

3.  Effect of crilvastatin, a new cholesterol lowering agent, on unesterified LDL-cholesterol metabolism into bile salts by rat isolated hepatocytes.

Authors:  T Clerc; V Sbarra; N Diaconescu; H Lafont; G Jadot; C Laruelle; F Chanussot
Journal:  Br J Pharmacol       Date:  1995-02       Impact factor: 8.739
  3 in total

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