| Literature DB >> 22639264 |
S Steven Potter1, Eric W Brunskill.
Abstract
This chapter describes detailed methods used for laser capture microdissection (LCM) of discrete subpopulations of cells. Topics covered include preparing tissue blocks, cryostat sectioning, processing slides, performing the LCM, and purification of RNA from LCM samples. Notes describe the fine points of each operation, which can often mean the difference between success and failure.Entities:
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Year: 2012 PMID： 22639264 PMCID： PMC5776720 DOI： 10.1007/978-1-61779-851-1_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745