| Literature DB >> 22639247 |
Xing Zhang1, Kevin T Bush, Sanjay K Nigam.
Abstract
In vitro culture of embryonic kidney rudiments has been utilized to study a variety of cellular processes and developmental mechanisms. Here, we describe two-dimensional (2D) culture of embryonic kidney rudiments on Transwell filters and three-dimensional (3D) cultures in collagen gels in detail, and 3D culture of isolated ureteric bud (UB) in Matrigel with BSN-conditioned media.Entities:
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Year: 2012 PMID: 22639247 DOI: 10.1007/978-1-61779-851-1_2
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745