PURPOSE: The edible mushroom (fungus) Agaricus blazei Murill (ABM) is a health food in many countries. Importantly, it has been shown to have antitumor and immune effects. There is no available information on ABM-affected immune responses in leukemia mice in vivo. Experimental Design. In this study, the authors investigated the immunopotentiating activities of boiled water-soluble extracts from desiccated ABM in WEHI-3 leukemia mice. The major characteristic of WEHI-3 leukemia mice are enlarged spleens and livers after intraperitoneal injection with murine leukemia WEHI-3 cells. Isolated T cells from spleens of ABM-treated mice resulted in increased T-cell proliferation compared with the untreated control with concanavalin A stimulation. RESULTS: ABM decreased the spleen and liver weights when compared with WEHI-3 leukemia mice and this effect was a dose-dependent response. ABM promoted natural killer cell activity and phagocytosis by macrophage/monocytes in leukemia mice in a dose-dependent manner. ABM also enhanced cytokines such as interleukin (IL)-1β, IL-6, and interferon-γ levels but reduced the level of IL-4 in WEHI-3 leukemia mice. Moreover, ABM increased the levels of CD3 and CD19 but decreased the levels of Mac-3 and CD11b in leukemia mice. CONCLUSIONS: The ABM extract is likely to stimulate immunocytes and regulate immune response in leukemia mice in vivo.
PURPOSE: The edible mushroom (fungus) Agaricus blazei Murill (ABM) is a health food in many countries. Importantly, it has been shown to have antitumor and immune effects. There is no available information on ABM-affected immune responses in leukemiamice in vivo. Experimental Design. In this study, the authors investigated the immunopotentiating activities of boiled water-soluble extracts from desiccated ABM in WEHI-3 leukemiamice. The major characteristic of WEHI-3 leukemiamice are enlarged spleens and livers after intraperitoneal injection with murineleukemia WEHI-3 cells. Isolated T cells from spleens of ABM-treated mice resulted in increased T-cell proliferation compared with the untreated control with concanavalin A stimulation. RESULTS:ABM decreased the spleen and liver weights when compared with WEHI-3 leukemiamice and this effect was a dose-dependent response. ABM promoted natural killer cell activity and phagocytosis by macrophage/monocytes in leukemiamice in a dose-dependent manner. ABM also enhanced cytokines such as interleukin (IL)-1β, IL-6, and interferon-γ levels but reduced the level of IL-4 in WEHI-3 leukemiamice. Moreover, ABM increased the levels of CD3 and CD19 but decreased the levels of Mac-3 and CD11b in leukemiamice. CONCLUSIONS: The ABM extract is likely to stimulate immunocytes and regulate immune response in leukemiamice in vivo.
Authors: Fhernanda R Smiderle; Cristiane H Baggio; Débora G Borato; Arquimedes P Santana-Filho; Guilherme L Sassaki; Marcello Iacomini; Leo J L D Van Griensven Journal: PLoS One Date: 2014-10-17 Impact factor: 3.240
Authors: Cynthia H Val; Fátima Brant; Aline S Miranda; Flávia G Rodrigues; Bruno C L Oliveira; Elândia A Santos; Diego R R Assis; Lísia Esper; Bruno C Silva; Milene A Rachid; Herbert B Tanowitz; Antônio L Teixeira; Mauro M Teixeira; Wiliam C B Régis; Fabiana S Machado Journal: Malar J Date: 2015-08-11 Impact factor: 2.979