Literature DB >> 22636011

White mutants of chloroperoxidase-secreting Caldariomyces fumago as superior production strains, revealing an interaction between pigmentation and enzyme secretion.

Markus Buchhaupt1, Sonja Hüttmann, Jens Schrader.   

Abstract

By mutant-colony screening of Caldariomyces fumago, several white mutants were isolated that are superior strains for the production of the valuable enzyme chloroperoxidase (CPO). Their culture supernatant lacks the contaminating dark pigment, which simplifies downstream processing. Furthermore, the CPO content increased significantly faster than the wild-type rate, which uncovers possible interactions between pigmentation and enzyme secretion.

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Year:  2012        PMID： 22636011      PMCID： PMC3406132          DOI： 10.1128/AEM.00570-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  5 in total

1.  Kinetics of cellulase inactivation by melanin.

Authors:  A T Bull
Journal:  Enzymologia       Date:  1970-12-30

2.  Melanin as a potential cryptococcal defence against microbicidal proteins.

Authors:  T L Doering; J D Nosanchuk; W K Roberts; A Casadevall
Journal:  Med Mycol       Date:  1999-06       Impact factor: 4.076

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Authors:  D R Morris; L P Hager
Journal:  J Biol Chem       Date:  1966-04-25       Impact factor: 5.157

4.  A defined growth medium for the production of chloroperoxidase by Caldariomyces fumago.

Authors:  M A Pickard
Journal:  Can J Microbiol       Date:  1981-12       Impact factor: 2.419

5.  Fructose induces and glucose represses chloroperoxidase mRNA levels.

Authors:  M J Axley; P Kenigsberg; L P Hager
Journal:  J Biol Chem       Date:  1986-11-15       Impact factor: 5.157
  5 in total
  3 in total

1.  Partial secretome analysis of Caldariomyces fumago reveals extracellular production of the CPO co-substrate H2O2 and provides a coproduction concept for CPO and glucose oxidase.

Authors:  Markus Buchhaupt; Karin Lintz; Sonja Hüttmann; Jens Schrader
Journal:  World J Microbiol Biotechnol       Date:  2018-01-10       Impact factor: 3.312

Review 2.  Identification and Expression of New Unspecific Peroxygenases - Recent Advances, Challenges and Opportunities.

Authors:  Alina Kinner; Katrin Rosenthal; Stephan Lütz
Journal:  Front Bioeng Biotechnol       Date:  2021-07-07

3.  Identification of a Caldariomyces fumago mutant secreting an inactive form of chloroperoxidase lacking the heme group and N-glycans.

Authors:  Sonja Hüttmann; Markus Buchhaupt; Jens Schrader
Journal:  PLoS One       Date:  2013-07-02       Impact factor: 3.240
  3 in total

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